Down-regulation of survivin suppresses uro-plasminogen activator through transcription factor JunB

Title
Down-regulation of survivin suppresses uro-plasminogen activator through transcription factor JunB
Author(s)
이경희최은영[최은영]고성애[고성애]김민경김경옥이시형장병익김상운송선교최준혁김재룡김세원
Keywords
HEPATOCYTE GROWTH-FACTOR; GASTRIC-CANCER; IN-VIVO; EXPRESSION; APOPTOSIS; GENE; PROTEIN; PROGNOSIS; CELLS; CARCINOMAS
Issue Date
201109
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Citation
EXPERIMENTAL AND MOLECULAR MEDICINE, v.43, no.9, pp.501 - 509
Abstract
Survivin, a member of the inhibitors of apoptosis protein family, is expressed during development and in various human cancers. However, the clinical relevance of survivin in cancer is still a matter of debate. Genes induced by hepatocyte growth factor (HGF) were screened using cDNA microarray technology in the stomach cancer cell lines, NUGC3 and MKN28. The levels of JunB, survivin, and uro-plasminogen activator (uPA) were up-regulated in cells treated with HGF in a dose-dependent manner. HGF-induced up regulation of JunB, survivin, and uPA was inhibited by pre-treatment with a MEK inhibitor (PD 98059). HGF-induced up-regulation of uPA was repressed by survivin knockdown. HGF enhanced the binding activity of JunB to the survivin promoter in control cells, but not in the JunB-shRNA cells. Transfection with survivin-shRNA resulted in a decrement of cell proliferation, as determined with MTT assays. In an in vitro invasion assay, significantly fewer cells transfected with survivin shRNA than control cells were able to invade across a Matrigel membrane barrier. In conclusion, survivin appeared to play an important role in the up-regulation of uPA induced by HGF via JunB and might contribute to HGF-mediated tumor invasion and metastasis, which may serve as a promising target for gastric cancer therapy.
URI
http://hdl.handle.net/YU.REPOSITORY/24642http://dx.doi.org/10.3858/emm.2011.43.9.057
ISSN
1226-3613
Appears in Collections:
의과대학 > 내과학교실 > Articles
의과대학 > 성형외과학교실 > Articles
의과대학 > 병리학교실 > Articles
의과대학 > 생화학.분자생물학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE