Manassantin A inhibits cAMP-induced melanin production by down-regulating the gene expressions of MITF and tyrosinase in melanocytes

Title
Manassantin A inhibits cAMP-induced melanin production by down-regulating the gene expressions of MITF and tyrosinase in melanocytes
Author(s)
손종근이화동이원희[이원희]노은미리[노은미리]서창섭이승호황방연[황방연]정상헌[정상헌]한상배[한상배]김영수[김영수]
Keywords
TRANSCRIPTION FACTOR; PROTEIN-KINASE; CYCLIC-AMP; MICROPHTHALMIA; MELANOGENESIS; PIGMENTATION; CELLS; BIOSYNTHESIS; MECHANISM; PROMOTER
Issue Date
201109
Publisher
WILEY-BLACKWELL
Citation
EXPERIMENTAL DERMATOLOGY, v.20, no.9, pp.761 - 763
Abstract
Microphthalmia-associated transcription factor (MITE) is inducible in response to cAMP through the cAMP-responsive element-binding protein (CREB) and plays a pivotal role in the melanocyte-specific expression of tyrosinase or tyrosinase-related proteins (TRPs) for melanin biosynthesis. Manassantin A from Saururus chinensis inhibits cAMP-induced melanin production in B16 melanoma cells. Here, we focused on molecular basis of the antimelanogenic activity. Manassantin A consistently inhibited the cAMP elevator 3-isobutyl-1-methylxanthine (IBMX)- or dibutyryl cAMP-induced melanin production in B16 cells or in melan-a melanocytes by down-regulating the expression of tyrosinase or TRP1 gene. Moreover, manassantin A suppressed MITF induction through IBMX-activated CREB pathway, directly inhibiting the Ser-133 phosphorylation of CREB. However, manassantin A did not affect IBMX-increased cAMP levels in these cells but also other cAMP-dependent melanogenic pathways through post-translational modifications of MITF. This putative molecular mechanism of manassantin A in the inhibition of melanin production suggests its pharmacological potential in skin hyperpigmentation.
URI
http://hdl.handle.net/YU.REPOSITORY/24609http://dx.doi.org/10.1111/j.1600-0625.2011.01296.x
ISSN
0906-6705
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약학대학 > 약학부 > Articles
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