Thermodynamic Investigation of the Formation of Complexes between Norfloxacin and Various Mononucleotides

Title
Thermodynamic Investigation of the Formation of Complexes between Norfloxacin and Various Mononucleotides
Author(s)
권용준[권용준]이현미[이현미]한성욱[한성욱]이동진[이동진]조태섭
Keywords
OPTICALLY-ACTIVE OFLOXACIN; DNA GYRASE; ENANTIOSELECTIVE BINDING; R-OFLOXACIN; QUINOLONE ANTIBACTERIALS; S-OFLOXACIN; INHIBITION; MECHANISM
Issue Date
201109
Publisher
KOREAN CHEMICAL SOC
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.32, no.9, pp.3233 - 3238
Abstract
The fluorescence of norfloxacin was quenched by various nucleotides. The ratio of the fluorescence intensities in the absence and presence of nucleotide was linearly dependent on nucleotide concentration, suggesting that quenching occurred through the formation of nonfluorescent norfloxacin-nucleotide complexes. The gradient of the linear relationship represented the equilibrium constant of complex formation; it decreased with increasing temperature. The slopes of van't Hoff plots constructed from the temperature-dependent equilibrium constants were positive in all cases, indicating that complex formation was energetically favorable - i.e., exothermic, with negative Gibb's free energy. The equilibrium constant increased when triphosphate was used instead of monophosphate. It also increased when the oxygen at the C'(2) position of the nucleotide was removed. Both enhancements were due to entropic effects: entropy decreased when complexes with AMP or GMP formed, while it increased when norfloxacin complexed with ATP, GTP, dAMP and dGMP.
URI
http://hdl.handle.net/YU.REPOSITORY/24602http://dx.doi.org/10.5012/bkcs.2011.32.9.3233
ISSN
0253-2964
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교육대학원 > 교육대학원 > Articles
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