Biosynthetic substitution of tyrosine in green fluorescent protein with its surrogate fluorotyrosine in Escherichia coli

Title
Biosynthetic substitution of tyrosine in green fluorescent protein with its surrogate fluorotyrosine in Escherichia coli
Author(s)
윤형돈니라이쿨람아야두레이사라바난[사라바난]카나가벨디판쿠마[카나가벨디판쿠마]김아란[김아란]이선구[이선구]
Keywords
SITE-SPECIFIC INCORPORATION
Issue Date
201109
Publisher
SPRINGER
Citation
BIOTECHNOLOGY LETTERS, v.33, no.11, pp.2201 - 2207
Abstract
Introduction of a fluorine moiety into green fluorescent protein offers an interesting novel spectral variant. The calculated binding energy of fluorotyrosine (F-Tyr) (-8.42 kcal/mol) for tyrosyl tRNA synthetase was moderately higher than that of tyrosine (Tyr) (-8.36 kcal/mol). This result directly correlated with the expression level of F-Tyr containing GFP (38 mg/l), which was comparably higher than that of the parent GFP expression level (34 mg/l). Finally, we generated a model structure for GFP to assess possible interaction in the chromophore of the protein structure, which plays an important role in determining the spectral and folding behaviors of the F-Tyr incorporated GFP variant.
URI
http://hdl.handle.net/YU.REPOSITORY/24594http://dx.doi.org/10.1007/s10529-011-0679-4
ISSN
0141-5492
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생명공학부 > 생명공학부 > Articles
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