A high-throughput dielectrophoresis-based cell electrofusion microfluidic device

Title
A high-throughput dielectrophoresis-based cell electrofusion microfluidic device
Author(s)
스즈첸Ning Hu[Ning Hu]Jun Yang[Jun Yang]Zheng-Qin Yin[Zheng-Qin Yin]Ye Ai[Ye Ai]Irina B. Svir[Irina B. Svir]Bin Xia[Bin Xia]Jia-Wen Yan[Jia-Wen Yan]Wen-Sheng Hou[Wen-Sheng Hou]Xiao-Lin Zheng[Xiao-Lin Zheng]
Keywords
EMBRYONIC STEM-CELLS; PROTOPLAST FUSION; SOMATIC-CELLS; CHIP; STRATEGIES; HYBRIDS
Issue Date
201109
Publisher
WILEY-BLACKWELL
Citation
ELECTROPHORESIS, v.32, no.18, pp.2488 - 2495
Abstract
A high-throughput cell electrofusion microfluidic chip has been designed, fabricated on a silicon-on-insulator wafer and tested for in vitro cell fusion under a low applied voltage. The developed chip consists of six individual straight microchannels with a 40-mu m thickness conductive highly doped Si layer as the microchannel wall. In each microchannel, there are 75 pairs of counter protruding microelectrodes, between which the cell electrofusion is performed. The entire highly doped Si layer is covered by a 2-mu m thickness aluminum film to maintain a consistent electric field between different protruding microelectrode pairs. A 150-nm thickness SiO(2) film is subsequently deposited on the top face of each protruding microelectrode for better biocompatibility. Owing to the short distance between two counter protruding microelectrodes, a high electric field can be generated for cell electrofusion with a low voltage imposed across the electrodes. Both mammalian cells and plant protoplasts were used to test the cell electrofusion. About 42-68% cells were aligned to form cell-cell pairs by the dielectrophoretic force. After cell alignment, cell pairs were fused to form hybrid cells under the control of cell electroporation and electrofusion signals. The averaged fusion efficiency in the paired cells is above 40% (the highest was about 60%), which is much higher than the traditional polyethylene glycol method (o5%) and traditional electrofusion methods (similar to 12%). An individual cell electrofusion process could be completed within 10 min, indicating a capability of high throughput.
URI
http://hdl.handle.net/YU.REPOSITORY/24582http://dx.doi.org/10.1002/elps.201100082
ISSN
0173-0835
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공과대학 > 기계공학부 > Articles
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