Anti-inflammatory Activity of 6-Hydroxy-2,7-dimethoxy-1,4-henanthraquinone from Tuberous Roots of Yam (Dioscorea batatas) through Inhibition of Prostaglandin D-2 and Leukotriene C-4 Production in Mouse Bone Marrow-derived Mast Cells

Title
Anti-inflammatory Activity of 6-Hydroxy-2,7-dimethoxy-1,4-henanthraquinone from Tuberous Roots of Yam (Dioscorea batatas) through Inhibition of Prostaglandin D-2 and Leukotriene C-4 Production in Mouse Bone Marrow-derived Mast Cells
Author(s)
장현욱Meihua Jin[Meihua Jin]양주혜[양주혜]조태형[조태형]박영인[박영인]이종길[이종길]박상조[박상조]손건호[손건호]유예[유예]
Keywords
NF-KAPPA-B; OCCUPATIONAL ASTHMA; ANTIOXIDANT ACTIVITIES; PINELLIA-TERNATA; DUAL INHIBITORS; RAW264.7 CELLS; 5-LIPOXYGENASE; EXPRESSION; CYCLOOXYGENASES-2; DIOSCIN
Issue Date
201109
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.34, no.9, pp.1495 - 1501
Abstract
6-Hydroxy-2,7-dimethoxy-1,4-phenanthraquinone (PAQ) isolated from the tuberous roots of Yam (Dioscorea batatas) inhibited cyclooxygenase-2 (COX-2) and cyclooxygenase-1 (COX-1) dependent prostaglandin D-2 (PGD(2)) generation in mouse bone marrow-derived mast cells in a concentration-dependent manner with IC50 values of 0.08 mu M and 0.27 mu M, respectively. In the Western blotting with specific anti-COX-2 antibodies, the decrease of the quantity of PGD(2) was accompanied by a decrease in the COX-2 protein level. But PAQ did not affect COX-1 protein level. In addition, this compound inhibited 5-lipoxygenase (5-LOX) dependent production of leukotriene C-4 in a dose-dependent manner, with an IC50 of 0.032 mu M. These results demonstrate that PAQ has a dual COX-2/5-LOX inhibitory activity. This compound also inhibited the degranulation reaction in a dose-dependent manner with an IC50 of 2.7 mu M. Thus, these results suggest that PAQ may be useful in regulating mast cell-mediated inflammatory diseases.
URI
http://hdl.handle.net/YU.REPOSITORY/24576http://dx.doi.org/10.1007/s12272-011-0911-8
ISSN
0253-6269
Appears in Collections:
약학대학 > 약학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE