Characterization of an Enzyme Capable of Degrading the Sheath of the Sphaerotilus natans from Terrabacter sp FK3

Title
Characterization of an Enzyme Capable of Degrading the Sheath of the Sphaerotilus natans from Terrabacter sp FK3
Author(s)
조윤래김병극[김병극]
Keywords
ACTIVATED-SLUDGE; FILAMENTOUS MICROORGANISMS; IDENTIFICATION; ORGANISMS
Issue Date
201110
Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
Citation
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.54, no.5, pp.750 - 757
Abstract
A microorganism for biological control of sludge bulking was isolated from soil. The isolated microorganism was able to degrade the sheath of the Sphaerotilus natans causing a bulking of activated sludge. The isolated strain, FK3, was identified as a Terrabacter species by analysis of its 16S rRNA genetic sequence. Glucose (0.1%, w/v) and 0.1% yeast extract as carbon and nitrogen sources, respectively, were used in the medium for Terrabacter sp. FK3 growth and enzyme production. Sheath-degrading crude enzyme was purified from a Terrabacter sp. FK3 culture broth via ultrafiltration, followed by DEAE-cellulose chromatography. Although the enzyme activity was optimal at pH 8 and 30 degrees C, the enzyme maintained stable activity below 50 degrees C and in the pH range from 5-10. Sheath-degrading enzyme activity was accelerated by addition of Mg(2+), Ca(2+), and Fe(2+), but inhibited by addition of Ag(2+) or Hg(2+). Catalysis was additionally promoted by addition of hydroxyurea, sodium azide, and iodoacetic acid, but inhibited by EDTA.
URI
http://hdl.handle.net/YU.REPOSITORY/24473http://dx.doi.org/10.3839/jksabc.2011.113
ISSN
1738-2203
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생명공학부 > 생명공학부 > Articles
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