Rapid detection of dysfunctional high-density lipoproteins using isoelectric focusing-based microfluidic device to diagnose senescence-related disease

Title
Rapid detection of dysfunctional high-density lipoproteins using isoelectric focusing-based microfluidic device to diagnose senescence-related disease
Author(s)
조경현장욱주심재술이동연김재룡Prashanta Dutta[Prashanta Dutta]
Keywords
APOLIPOPROTEIN-A-I; NONENZYMATIC GLYCATION; METABOLIC SYNDROME; END-PRODUCTS; APOA-I; CHOLESTEROL; PROTEINS; FRUCTOSE; CAPILLARY; CHIP
Issue Date
201111
Publisher
WILEY-BLACKWELL
Citation
ELECTROPHORESIS, v.32, no.23, pp.3415 - 3423
Abstract
Recently, we reported elevated levels of advanced glycated end products (AGEs) in human high-density lipoproteins (HDL), with fragmentation of apoA-I in an elderly group, compared with a younger group. More dysfunctional HDL from human plasma was demonstrated in the elderly group, including reconstituted HDL containing glycated apoA-I (gA-I-rHDL) with elevation of AGEs. Based on SDS-PAGE analysis, HDL3 from the elderly group (E-HDL3) exhibited increased multimerization with increased smear band intensity compared to HDL3 from the younger group (Y-HDL3). According to isoelectric focusing gel analysis, gA-I-rHDL and E-HDL3 showed electromobility to the basic region of pH with a broader band range. In a microfluidic channel, E-HDL3 had faster mobility with a broader range and a higher isoelectric point (pI, approximately 8.1), whereas Y-HDL3 showed a narrow band range with a lower pI (approximately 6.9). In conclusion, gA-I-rHDL and E-HDL share several electrophoretic properties with multimerization and faster mobility in microfluidic channels, depending on the isoelectric point. These results can be applied to develop a rapid detection system for modified HDL to predict the extent of aging and aging-related metabolic diseases, such as cardiovascular disease and diabetes.
URI
http://hdl.handle.net/YU.REPOSITORY/24280http://dx.doi.org/10.1002/elps.201100361
ISSN
0173-0835
Appears in Collections:
생명공학부 > 생명공학부 > Articles
공과대학 > 기계공학부 > Articles
의과대학 > 생화학.분자생물학교실 > Articles
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