Molecular cloning and characterization of the STAT gene in Hyphantria cunea haemocytes

Title
Molecular cloning and characterization of the STAT gene in Hyphantria cunea haemocytes
Author(s)
강영진서숙재[서숙재]한연수[한연수]진병래[진병래]이인희[이인희]김홍자[김홍자]김용일[김용일]권용민[권용민]전향미[전향미]
Keywords
BLOOD-CELL ACTIVATION; KAPPA-B FACTOR; IMMUNE-RESPONSE; PEPTIDOGLYCAN RECOGNITION; DROSOPHILA-MELANOGASTER; TRANSCRIPTIONAL CONTROL; SIGNALING PATHWAYS; ANOPHELES-GAMBIAE; MEDFLY HEMOCYTES; INNATE IMMUNITY
Issue Date
201112
Publisher
WILEY-BLACKWELL
Citation
INSECT MOLECULAR BIOLOGY, v.20, no.6, pp.723 - 732
Abstract
A new insect member of the signal transducer and activator of transcription (STAT) family of transcription factors, Hyphantria cunea STAT (HcSTAT), was cloned from the lepidopteran H. cunea. The domain involved in DNA interaction and the Src homology 2 (SH2) domain were well conserved. During all developmental stages, the gene was expressed at a low level in the haemocytes, fat body cells, midgut, epidermis and Malpighian tubules. The haemocytes and Malpighian tubules showed transcriptional activation of HcSTAT upon Gram-negative and Gram-positive bacterial challenges. These challenges increased the induction and nuclear translocation of the HcSTAT protein that recognizes a STAT target site in H. cunea haemocytes. In vivo treatment with sodium orthovanadate translocated HcSTAT to the haemocyte nucleus. This study shows the involvement of the haemocyte Janus kinase/STAT pathway after microbial infection in lepidopteran insects.
URI
http://hdl.handle.net/YU.REPOSITORY/24218http://dx.doi.org/10.1111/j.1365-2583.2011.01102.x
ISSN
0962-1075
Appears in Collections:
의과대학 > 약리학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE