Prevalence of Plasmid-Mediated Quinolone Resistance and Mutations in the Gyrase and Topoisomerase IV Genes in Salmonella Isolated from 12 Tertiary-Care Hospitals in Korea

Title
Prevalence of Plasmid-Mediated Quinolone Resistance and Mutations in the Gyrase and Topoisomerase IV Genes in Salmonella Isolated from 12 Tertiary-Care Hospitals in Korea
Author(s)
이채훈정행순[정행순]김정아[김정아]신정환[신정환]장철훈[장철훈]정윤섭[정윤섭]조지현[조지현]김미나[김미나]김영리[김영리]이경원[이경원]이미애[이미애]이위교[이위교]신종희[신종희]이정녀[이정녀]
Keywords
ESCHERICHIA-COLI; QNR GENES; TRANSFERABLE PLASMID; MODIFYING ENZYME; UNITED-STATES; ENTERICA; MECHANISMS; TYPHIMURIUM; EMERGENCE; SEROTYPES
Issue Date
201112
Publisher
MARY ANN LIEBERT INC
Citation
MICROBIAL DRUG RESISTANCE, v.17, no.4, pp.551 - 557
Abstract
Background: The aim of this study was to investigate the prevalence of plasmid-mediated quinolone resistance (PMQR) and mutations in quinolone resistance-determining regions (QRDRs) of Salmonella and their association with fluoroquinolone susceptibility in Korea. Methods: A total of 284 nonduplicated clinical isolates of Salmonella were collected from various clinical specimens at 12 tertiary-care hospitals in Korea. The qnrA, qnrB, and qnrS genes were detected by multiplex polymerase chain reaction (PCR). The qepA and aac(6')-Ib-cr genes were amplified by PCR. The QRDRs of gyrA, gyrB, parC, and parE were amplified by PCR from the DNA of selected nalidixic acid-resistant and qnr-positive isolates. Results: We detected six qnr-positive Salmonella (four qnrS1 and two qnrB19) and one aac(6')-Ib-cr-positive strain. A mutation in the QRDR of gyrA only (N = 46) was the most common, followed by gyrA + parC (N = 9), parC (N = 7), gyrA + parE (N = 3), parC + parE (N = 3), gyrA + gyrB (N = 2), and parE (N = 1). There were seven novel mutations in the QRDR regions of gyrB, parC, and parE. Six of seven PMQR-positive isolates had high-level resistance to nalidixic acid, and all six strains had reduced susceptibility to ciprofloxacin. One qnrS1-positive isolate was resistant to ciprofloxacin, norfloxacin, and nalidixic acid. The resistant rates to nalidixic acid, ciprofloxacin, norfloxacin, and levofloxacin were 49.3%, 1.1%, 0.7%, and 0.4%, respectively. Conclusion: We report the first detection of PMQR in Salmonella isolates from Korea. It is essential to continue surveillance and to watch for the spread of PMQR in Salmonella for public health control.
URI
http://hdl.handle.net/YU.REPOSITORY/24215http://dx.doi.org/10.1089/mdr.2011.0095
ISSN
1076-6294
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의과대학 > 진단검사의학교실 > Articles
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