Soybean mosaic virus Infection and Helper Component-protease Enhance Accumulation of Bean pod mottle virus-Specific siRNAs

Title
Soybean mosaic virus Infection and Helper Component-protease Enhance Accumulation of Bean pod mottle virus-Specific siRNAs
Author(s)
임현섭[임현섭]장찬용[장찬용]배한홍김준기[김준기]이철호[이철호]홍진성[홍진성]주호종[주호종]김홍기[김홍기]도마이어 레슬리[도마이어 레슬리]
Keywords
RNA SILENCING SUPPRESSORS; HC-PRO; VIRAL SUPPRESSORS; SYMPTOM DEVELOPMENT; PLANT-VIRUSES; EXPRESSION; PATHOGENICITY; STRATEGY; RECOVERY; TOBACCO
Issue Date
201112
Publisher
KOREAN SOC PLANT PATHOLOGY
Citation
PLANT PATHOLOGY JOURNAL, v.27, no.4, pp.315 - 323
Abstract
Soybean plants infected with Bean pod mottle virus (BPMV) develop acute symptoms that usually decrease in severity over time. In other plant-virus interactions, this type of symptom recovery has been associated with degradation of viral RNAs by RNA silencing, which is accompanied by the accumulation of virus-derived small interfering RNAs (siRNAs). In this study, changes in the accumulation of BPMV siRNAs were investigated in soybean plants infected with BPMV alone, or infected with both BPMV and Soybean mosaic virus (SMV) and in transgenic soybean plants expressing SMV helper component-protease (HC-Pro). In many potyviruses, HC-Pro is a potent suppressor of RNA silencing. In plants infected with BPMV alone, accumulation of siRNAs was positively correlated with symptom severity and accumulation of BPMV genomic RNAs. Plants infected with both BPMV and SMV and BPMV-infected transgenic soybean plants expressing SMV HC-Pro exhibited severe symptoms characteristic of BPMV-SMV synergism, and showed enhanced accumulation of BPMV RNAs and siRNAs compared to plants infected with BPMV alone and nontransgenic plants. Likewise, SMV HC-Pro enhanced the accumulation of siRNAs produced from a silenced green fluorescent protein gene in transient expression assays, while the P19 silencing suppressor of Tomato bushy stunt virus did not. Consistent with the modes of action of HC-Pro in other systems, which have shown that HC-Pro suppresses RNA silencing by preventing the unwinding of duplex siRNAs and inhibiting siRNA methylation, these studies showed that SMV HC-Pro interfered with the activities of RNA-induced silencing complexes, but not the activities of Dicer-like enzymes in antiviral defenses.
URI
http://hdl.handle.net/YU.REPOSITORY/24213http://dx.doi.org/10.5423/PPJ.2011.27.4.315
ISSN
1598-2254
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생명공학부 > 생명공학부 > Articles
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