The Effect of Doxycycline on PMA-Induced MUC5B Expression via MMP-9 and p38 in NCI-H292 Cells

Title
The Effect of Doxycycline on PMA-Induced MUC5B Expression via MMP-9 and p38 in NCI-H292 Cells
Author(s)
김용대배창훈천승민[천승민]이흥만[이흥만]송시연
Keywords
AIRWAY EPITHELIAL-CELLS; INDUCED MUCIN PRODUCTION; GENE-EXPRESSION; MATRIX-METALLOPROTEINASE-9; DIFFERENTIATION; COPD; ERK
Issue Date
201112
Publisher
KOREAN SOC OTORHINOLARYNGOL
Citation
CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, v.4, no.4, pp.177 - 183
Abstract
Objectives. Doxycycline is commonly used in medicine for its bacteriostatic antimicrobial properties. Recent studies have reported that doxycycline also has anti-inflammatory effects. Matrix metalloproteinase (MMP)-9 has been found to be involved in the physiological and pathological process of inflammatory airway disease. Phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, is known to stimulate the expression of MMP and mucin genes in the airway and intestinal epithelial cells, Therefore, the effects and signal pathways of doxycycline on PMA-induced MUC5B expression dependent MMP-9 in human airway epithelial cells were investigated. Methods. In human NCI-H292 airway epithelial cells, MUC5B and MMP-9 mRNA expression, MUC5B protein expression, and MMP-9 protein activity after the treatment with PMA, MMP-9 or doxycycline were determined by reverse transcriptase-polymerase chain reaction, enzyme immunoassay, gelatin zymography, and Western blot analysis. Results. PMA increased MMP-9 and MUC5B expression. MMP-9 increased MUC5B expression. Doxycycline inhibited PMA-induced MUC5B expression, and PMA-induced MMP-9 mRNA expression and protein activity Doxycycline inhibited phosphorylation of p38 induced by PMA and MMP-9. Conclusion. The results of this study suggest that doxycycline inhibited PMA-induced MUC5B mRNA expression and protein production through the MMP-9 and p38 pathways in human NCI-H292 airway epithelial cells.
URI
http://hdl.handle.net/YU.REPOSITORY/24210http://dx.doi.org/10.3342/ceo.2011.4.4.177
ISSN
1976-8710
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의과대학 > 이비인후과학교실 > Articles
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