Saponins from the roots of Platycodon grandiflorum suppress ultraviolet A-induced matrix metalloproteinase-1 expression via MAPKs and NF-kappa B/AP-1-dependent signaling in HaCaT cells

Title
Saponins from the roots of Platycodon grandiflorum suppress ultraviolet A-induced matrix metalloproteinase-1 expression via MAPKs and NF-kappa B/AP-1-dependent signaling in HaCaT cells
Author(s)
황용필[황용필]김형균[김형균]최재호[최재호]한은희[한은희]권광일[권광일]이영춘[이영춘]최준민[최준민]정영철[정영철]정태천정혜광[정혜광]
Keywords
NF-KAPPA-B; HUMAN-SKIN; HUMAN KERATINOCYTES; DERMAL FIBROBLASTS; RAW-264.7 CELLS; NITRIC-OXIDE; TUMOR-CELLS; IRRADIATION; INHIBITION; PATHWAY
Issue Date
201112
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.49, no.12, pp.3374 - 3382
Abstract
Saponins from the roots of Platycodon grandiflorum (CKS) have been shown to exhibit many pharmacological activities, including anti-cancer and anti-inflammatory activities and antioxidant effects. However, anti-skin photoaging effects of CKS have not yet been reported. In this study, we investigated the protective effects of CKS against UVA damage on immortalized human keratinocytes (HaCaT). We then explored the inhibitory effects of CKS on UVA-induced MMP-1 and investigated the molecular mechanism underlying those effects. CKS increased the cell viability and inhibited reactive oxygen species (ROS) production in HaCaT cells exposed to UVA irradiation. Pre-treatment of HaCaT cells with CKS inhibited UVA-induced production of MMP-1 and MMP-9. In addition, CKS decreased UVA-induced expression of the inflammatory cytokines IL-1 beta and IL-6. Western blot analysis further revealed that CKS markedly suppressed the enhancement of collagen degradation in UVA-exposed HaCaT cells. CKS also suppressed UVA-induced activation of NF-kappa B or c-Jun and c-Fos, and the phosphorylation of MAPKs, which are upstream modulators of NF-kappa B and AP-1. (C) 2011 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/24194http://dx.doi.org/10.1016/j.fct.2011.10.002
ISSN
0278-6915
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약학대학 > 약학부 > Articles
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