Fecal Colonization of Enterobacteriaceae Carrying Plasmid-Mediated Quinolone Resistance Determinants in Korea

Title
Fecal Colonization of Enterobacteriaceae Carrying Plasmid-Mediated Quinolone Resistance Determinants in Korea
Author(s)
이채훈정행순[정행순]배일권[배일권]신정환[신정환]김시현[김시현]장철훈[장철훈]정윤성[정윤성]김선주[김선주]류남희[류남희]이정녀[이정녀]
Keywords
ESCHERICHIA-COLI; KLEBSIELLA-PNEUMONIAE; UNITED-STATES; TRANSFERABLE PLASMID; MODIFYING ENZYME; QNR GENES; PREVALENCE; AAC(6')-IB-CR; QEPA; EPIDEMIOLOGY
Issue Date
201112
Publisher
MARY ANN LIEBERT INC
Citation
MICROBIAL DRUG RESISTANCE, v.17, no.4, pp.507 - 512
Abstract
The aims of the current study were to investigate the prevalence and molecular characteristics of plasmid-mediated quinolone resistance (PMQR) genes from colonizing fecal organisms and to compare the incidence and subtype of these genes according to bacterial species and hospital at five tertiary-care hospitals in Korea. A total of 500 nonduplicated clinical isolates of Enterobacteriaceae were obtained from fecal specimens at five tertiary-care hospitals between March and May 2008. The PMQR genes (qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA) were amplified by PCR and confirmed by direct sequencing of the PCR products. A total of 83 (16.6%) qnr-positive isolates were detected. The prevalence rates of qnrA, qnrB, and qnrS were 1.4%, 13.6%, and 1.6%, respectively. The species distributions of qnrB-positive isolates were Klebsiella pneumoniae (37/109; 33.9%), Citrobacter freundii (10/34; 29.4%), Citrobacter braakii (8/13; 61.5%), and Escherichia coli (8/275; 2.9%). Sixteen subtypes of qnrB were detected, including seven novel variants. The prevalences of aac(6')-Ib-cr and qepA were 15.6% (n = 78) and 0.6% (n = 3), respectively. The aac(6')-Ib-cr gene was detected in 39 (47.0%) of 83 qnr-positive isolates and 39 (9.4%) of 417 qnr-negative isolates There was one qepA variant containing a novel mutation (Ala231Val). The prevalence of PMQR genes was high in Enterobacteriaceae from stool specimens in Korea, and there was a close relation between qnr and aac(6')-Ib-cr.
URI
http://hdl.handle.net/YU.REPOSITORY/24183http://dx.doi.org/10.1089/mdr.2011.0040
ISSN
1076-6294
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의과대학 > 진단검사의학교실 > Articles
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