Angiotensin II inhibits chemokine CCL5 expression in vascular smooth muscle cells from spontaneously hypertensive rats

Title
Angiotensin II inhibits chemokine CCL5 expression in vascular smooth muscle cells from spontaneously hypertensive rats
Author(s)
김희선윤영현[윤영현]도병수김혜영
Keywords
ACTIVATED-PROTEIN-KINASE; FACTOR-KAPPA-B; GLOMERULAR ENDOTHELIAL-CELLS; UP-REGULATION; 12-LIPOXYGENASE ACTIVITY; DIFFERENTIAL ACTIVATION; RANTES; TRANSCRIPTION; RECEPTOR; INFLAMMATION
Issue Date
201112
Publisher
NATURE PUBLISHING GROUP
Citation
HYPERTENSION RESEARCH, v.34, no.12, pp.1313 - 1320
Abstract
Angiotensin II (Ang II) exerts some of its effects on the vasculature by stimulating chemokines and 12-lipoxygenase (12-LO). In addition, a high expression of chemokines by Ang II has been observed in vascular smooth muscle cells (VSMCs) in spontaneously hypertensive rats (SHR). In this study, the action mechanism of Ang II on CCL5 expression in SHR VSMCs was examined. Expression of CCL5 in SHR thoracic aorta tissues and VSMCs was lower than that in normotensive Wistar-Kyoto rats (WKY) thoracic aorta tissues and VSMCs. Moreover, Ang II inhibited CCL5 expression in SHR VSMCs, but not in WKY VSMCs. Inhibition of CCL5 by Ang II was mediated by both Ang II subtype 1 receptor (AT(1)R) and subtype 2 receptor (AT(2)R) activation in SHR VSMCs. However, Ang II did not inhibit CCL5 expression in SHR VSMCs that were transfected with 12-LO small interfering RNA. In addition, 12-LO metabolite, 12(S)-hydroxyeicosatetraenoic acid (HETE) inhibited CCL5 mRNA expression in SHR VSMCs. The expression of Ang II-induced 12-LO was also blocked by both AT(1)R and AT(2)R inhibitors. Mitogen-activated protein (MAP) kinase, extracellular signal-regulated kinase (ERK)1/2, p38 and Jun N-terminal kinase pathways all mediated the inhibitory action of Ang II on CCL5 expression in SHR VSMCs. Taken together, the inhibitory action of Ang II on CCL5 expression was shown to be mediated by the 12-LO pathway through the activation of both of AT(1)R and AT(2)R and this process was associated with MAP kinase pathways in SHR VSMCs. This result suggests that upregulation of 12-LO by Ang II leads to the downregulation of CCL5 expression in SHR VSMCs. Hypertension Research (2011) 34, 1313-1320; doi:10.1038/hr.2011.132; published online 4 August 2011
URI
http://hdl.handle.net/YU.REPOSITORY/24178http://dx.doi.org/10.1038/hr.2011.132
ISSN
0916-9636
Appears in Collections:
의과대학 > 미생물학교실 > Articles
의과대학 > 응급의학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE