A zebrafish model for the rapid evaluation of pro-oxidative and inflammatory death by lipopolysaccharide, oxidized low-density lipoproteins, and glycated high-density lipoproteins

Title
A zebrafish model for the rapid evaluation of pro-oxidative and inflammatory death by lipopolysaccharide, oxidized low-density lipoproteins, and glycated high-density lipoproteins
Author(s)
조경현박기훈
Keywords
APOLIPOPROTEIN-A-I; METABOLIC SYNDROME; DIETARY FRUCTOSE; DRUG DISCOVERY; APOA-I; ATHEROSCLEROSIS; CHOLESTEROL; SENESCENCE; HDL
Issue Date
201112
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation
FISH & SHELLFISH IMMUNOLOGY, v.31, no.6, pp.904 - 910
Abstract
Oxidation and inflammation are leading causes of nearly all chronic metabolic disorders, and play major roles in cardiovascular disease, cancer, and chronic age-dependent disease. High-density lipoprotein (HDL) and apolipoprotein (apo) A-I have strong antioxidant and anti-inflammatory properties in the plasma. Fructose-induced non-enzymatic glycation of apoA-I can lead to the production of dysfunctional apoA-I and HDL. To compare the physiologic effects of dysfunctional apoA-I and HDL, reconstituted HDL containing native apoA-I (nA-I) or glycated apoA-I (gA-I) was injected into zebrafish embryos in the presence of inflammatory molecules. Co-injection of reconstituted HDL containing VLDL and LDL gA-I (gA-I-rHDL) and lipopolysaccaride (LPS) resulted in acute embryo deaths, while rHDL containing nA-I (nA-I-rHDL) and LPS resulted in significantly enhanced survival. Co-injection of oxidized LDL (oxLDL) and nA-I-rHDL improved embryo survival, while co-injection of oxLDL and gA-I-rHDL aggravated inflammatory deaths. Furthermore, co-injection of oxLDL and HDL(2) (5 ng of protein) or HDL(3) (15 ng of protein) from the young group (22 +/- 2 years old) showed significantly increased embryo survival compared with the same co-injection of HDL from the elderly group (71 +/- 4 years old). In conclusion, our assay system provides a rapid and economic method to screen antioxidant and anti-inflammatory agents using zebrafish embryos. (C) 2011 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/24161http://dx.doi.org/10.1016/j.fsi.2011.08.006
ISSN
1050-4648
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생명공학부 > 생명공학부 > Articles
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