Sequencing and characterization of Varicella-Zoster virus vaccine strain SuduVax

Title
Sequencing and characterization of Varicella-Zoster virus vaccine strain SuduVax
Author(s)
박호선이찬희[이찬희]김종익[김종익]안진현[안진현]윤 엽[윤 엽]박송용[박송용]김근희[김근희]권시내[권시내]정규승[정규승]김유영[김유영]지가영[지가영]왕웬단[왕웬단]이건명[이건명]
Keywords
SINGLE NUCLEOTIDE POLYMORPHISMS; COMPLETE DNA-SEQUENCES; OKA VACCINE; CLADES; VZV; IDENTIFICATION; REPLICATION; GENOME
Issue Date
201112
Publisher
BIOMED CENTRAL LTD
Citation
VIROLOGY JOURNAL, v.8
Abstract
Background: Varicella-zoster virus (VZV) causes chickenpox in children and shingles in older people. Currently, live attenuated vaccines based on the Oka strain are available worldwide. In Korea, an attenuated VZV vaccine has been developed from a Korean isolate and has been commercially available since 1994. Despite this long history of use, the mechanism for the attenuation of the vaccine strain is still elusive. We attempted to understand the molecular basis of attenuation mechanism by full genome sequencing and comparative genomic analyses of the Korean vaccine strain SuduVax. Results: SuduVax was found to contain a genome that was 124,759 bp and possessed 74 open reading frames (ORFs). SuduVax was genetically most close to Oka strains and these Korean-Japanese strains formed a strong clade in phylogenetic trees. SuduVax, similar to the Oka vaccine strains, underwent T- > C substitution at the stop codon of ORF0, resulting in a read-through mutation to code for an extended form of ORF0 protein. SuduVax also shared certain deletion and insertion mutations in ORFs 17, 29, 56 and 60 with Oka vaccine strains and some clinical strains. Conclusions: The Korean VZV vaccine strain SuduVax is genetically similar to the Oka vaccine strains. Further comparative genomic and bioinformatics analyses will help to elucidate the molecular basis of the attenuation of the VZV vaccine strains.
URI
http://hdl.handle.net/YU.REPOSITORY/24149http://dx.doi.org/10.1186/1743-422X-8-547
ISSN
1743-422X
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의과대학 > 미생물학교실 > Articles
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