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dc.contributor.author임현섭[임현섭]ko
dc.contributor.author아나마리아 바이라[아나마리아 바이라]ko
dc.contributor.author배한홍ko
dc.contributor.author브래그[브래그]ko
dc.contributor.author루진[루진]ko
dc.contributor.author보캔[보캔]ko
dc.contributor.author다이넬트[다이넬트]ko
dc.contributor.author오웬[오웬]ko
dc.contributor.author해몬드[해몬드]ko
dc.date.accessioned2015-12-17T00:42:59Z-
dc.date.available2015-12-17T00:42:59Z-
dc.date.created2015-11-13-
dc.date.issued201008-
dc.identifier.citationJOURNAL OF GENERAL VIROLOGY, v.91, pp.2102 - 2115-
dc.identifier.issn0022-1317-
dc.identifier.urihttp://hdl.handle.net/YU.REPOSITORY/23875-
dc.identifier.urihttp://dx.doi.org/10.1099/vir.0.019448-0-
dc.description.abstractCell-to-cell movement of potexviruses requires coordinated action of the coat protein and triple gene block (TGB) proteins. The structural properties of Alternanthera mosaic virus (AltMV) TGB3 were examined by methods differentiating between signal peptides and transmembrane domains, and its subcellular localization was studied by Agrobacterium-mediated transient expression and confocal microscopy. Unlike potato virus X (PVX) TGB3, AltMV TGB3 was not associated with the endoplasmic reticulum, and accumulated preferentially in mesophyll cells. Deletion and site-specific mutagenesis revealed an internal signal VL(17,18) of TGB3 essential for chloroplast localization, and either deletion of the TGB3 start codon or alteration of the chloroplast-localization signal limited cell-to-cell movement to the epidermis, yielding a virus that was unable to move into the mesophyll layer. Overexpression of AltMV TGB3 from either AltMV or PVX infectious clones resulted in veinal necrosis and vesiculation at the chloroplast membrane, a cytopathology not observed in wild-type infections. The distinctive mesophyll and chloroplast localization of AltMV TGB3 highlights the critical role played by mesophyll targeting in virus long-distance movement within plants.-
dc.language영어-
dc.publisherSOC GENERAL MICROBIOLOGY-
dc.subjectGENE BLOCK PROTEINS-
dc.subjectFLUORESCENT PROTEIN-
dc.subjectHYDROPHOBIC SEGMENT-
dc.subjectSYSTEMIC MOVEMENT-
dc.subjectENCODED PROTEINS-
dc.subjectIN-VITRO-
dc.subjectX TGBP3-
dc.subjectRNA-
dc.subjectPOTEXVIRUS-
dc.subjectBARLEY-
dc.titleMutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement-
dc.typeArticle-
dc.identifier.wosid000280928500026-
dc.identifier.scopusid2-s2.0-77955302691-
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