Liquid chromatography-tandem mass spectrometry quantification of levosulpiride in human plasma and its application to bioequivalence study

Title
Liquid chromatography-tandem mass spectrometry quantification of levosulpiride in human plasma and its application to bioequivalence study
Author(s)
임미선Prasad B. Phapale[Prasad B. Phapale]이혜원[이혜원]성숙진[성숙진]김은희[김은희]박정현[박정현]이미란[이미란]황성규[황성규]윤영란[윤영란]
Keywords
THROUGHPUT QUANTITATIVE BIOANALYSIS; SULPIRIDE; MS/MS; PHARMACOKINETICS; METABOLITES; URINE; SERUM
Issue Date
201008
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.878, no.24, pp.2280 - 2285
Abstract
An improved method for determining levels of levosulpiride in human plasma using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed and validated. The protein precipitation method was used for plasma sample preparation. Levosulpiride and an internal standard (IS) were isocratically separated on a UPLC BEN C(18) column with a mobile phase of ammonium formate buffer (1 mM, adjusted to pH 3 with formic acid) and acetonitrile (60:40, v/v). MS/MS detection was performed by monitoring the parent -> daughter pair of levosulpiride and the IS at m/z 342 -> 112 and 329 -> 256, respectively. The method was linear from 2.5 to 200 ng/mL and exhibited acceptable precision and percent recovery. The method was successfully demonstrated in pharmacokinetic and bioequivalence studies of two levosulpiride oral formulations administered to healthy volunteers. When compared to the previous LC-MS methods, the proposed method is faster, well-validated, and uses lesser plasma volume and a similar sensitivity. The use of UPLC allowed rapid and sensitive quantification of levosulpiride, making this method suitable for high-throughput clinical applications. (C) 2010 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/23838http://dx.doi.org/10.1016/j.jchromb.2010.06.036
ISSN
1570-0232
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약학대학 > 약학부 > Articles
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