Powder Formulation Using Heat Resistant Endospores of Two Multi-functional Plant Growth Promoting Rhizobacteria Bacillus Strains Having Phytophtora Blight Suppression and Growth Promoting Functions

Title
Powder Formulation Using Heat Resistant Endospores of Two Multi-functional Plant Growth Promoting Rhizobacteria Bacillus Strains Having Phytophtora Blight Suppression and Growth Promoting Functions
Author(s)
김상달정수희임종희
Keywords
SUBTILIS AH18; BIOLOGICAL-CONTROL
Issue Date
201008
Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
Citation
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.53, no.4, pp.485 - 492
Abstract
Multi-functional plant growth promoting rhizobacteria Bacillus subtilis AH18 and Bacillus licheniformis K11 have been shown to display synergic effects in promoting the growth of red peppers and in suppressing phytophtora blight and have a competitive relationship in a limited nutritional environment. In the present study, a powder formulation method using a commercially cheap and easily obtainable carrier was developed to formulate these excellent antagonistic Bacillus species into a product. When the endospores of both strains were simultaneously formulated using a synthetic zeolite of NaY and natural zeolite, the growth rate of the vegetative form of B. sublitis AH18 was 5x10(9) CFU/mL. In contrast, the;growth of B. licheniformis K11 was suppressed when this formulation was used. However, when the synthetic zeolite of NaA was used in the simultaneous formulation, the survival rates of both bacteria at 45 degrees C for 60 days was sustained and the bacterial concentration was the same as the initial injection concentration of 5x10(9) CFU/mL. Furthermore, when the growth rates of the liquid state endospore preparations at different pH values were examined for 60 days, the concentration of both the Bacillus species were shown to slowly decrease at pH 5.0 and pH 9.0, but under strong basic conditions (pH 11.0), both the bacteria maintained stable endospore counts. When the effects of the prototype formulated using the NaA carrier at pH 11.0 was investigated in in viva pot tests, the formulation was shown to completely suppress the phytophtora blight and have an excellent growth promoting effect. Both the Bacillus species colonized the rhizosphere of red peppers for 15 days with more than 10(8) CFU/g. Therefore, the carrier formulation method developed in the present study using Bacillus endospores will allow for long storage of the product and can eliminate the competitive relationship between the two Bacillus species. In addition, if the formulation is commercially applied, several antagonistic microorganisms can be formulated into a single product, which is expected to be convenient for the manufacturing processes as well as increase the stability of the biological control agents for use in farmhouses.
URI
http://hdl.handle.net/YU.REPOSITORY/23826http://dx.doi.org/10.3839/jksabc.2010.074
ISSN
1738-2203
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생명공학부 > 생명공학부 > Articles
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