Effect of high hydrostatic pressure on the enzyme activities in Saccharomyces cerevisiae and Escherichia coli

Title
Effect of high hydrostatic pressure on the enzyme activities in Saccharomyces cerevisiae and Escherichia coli
Author(s)
방우석정현정[정현정]
Keywords
API ZYM; INACTIVATION; PECTINESTERASE; KINETICS; BACTERIA; CHEESE; SYSTEM; ORANGE
Issue Date
201009
Publisher
ELSEVIER SCIENCE BV
Citation
NEW BIOTECHNOLOGY, v.27, no.4, pp.440 - 444
Abstract
The purpose of this study was to evaluate the effect of high hydrostatic pressure (HHP) on the enzyme activities in Saccharomyces cerevisiae (ATCC 16664) and Escherichia coli (ATCC 11229). Enzyme activities before and after HHP treatment were determined using an APIZYME (R) enzyme assay kit. Thirteen active enzymes were detected in S. cerevisiae and E. coli. Pressure treatment at 448 MPa for 30 s at 23 degrees C resulted in different effects on enzymes in S. cerevisiae and E. con. HHP completely inactivated lipase, cystine arylamidase, and chymotrypsin and moderately inactivated esterase, esterase lipase, leucine arylamidase, valine arylamidase and alpha-glucosidase in S. cerevisiae. In E. coli, esterase, esterase lipase, lipase, valine arylamidase, cystine arylamidase, trypsin, alpha-glucosidase, and beta-glucuronidase were completely inactivated and leucine arylamidase and beta-galactosidase retained partial activities. Phosphoric hydrolases were not inactivated in both microorganisms. The use of the enzyme assay kit provided rapid and useful information on the microorganisms' enzymes and their sensitivity to HHP treatment in a simple manner.
URI
http://hdl.handle.net/YU.REPOSITORY/23666http://dx.doi.org/10.1016/j.nbt.2010.03.001
ISSN
1871-6784
Appears in Collections:
생활과학대학 > 식품영양학과 > Articles
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