Dimethyl sulfoxide elevates hydrogen peroxide-mediated cell death in Saccharomyces cerevisiae by inhibiting the antioxidant function of methionine sulfoxide reductase A

Title
Dimethyl sulfoxide elevates hydrogen peroxide-mediated cell death in Saccharomyces cerevisiae by inhibiting the antioxidant function of methionine sulfoxide reductase A
Author(s)
김화영곽근희최승희
Keywords
OXIDATIVE STRESS; R-SULFOXIDE; PROTEIN-CARBONYL; LIFE-SPAN; MSRA; MAMMALS; OVEREXPRESSION; RESISTANCE; DAMAGE; EXPRESSION
Issue Date
201009
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Citation
BMB REPORTS, v.43, no.9, pp.622 - 628
Abstract
Dimethyl sulfoxide (DMSO) can be reduced to dimethyl sulfide by MsrA, which stereospecifically catalyzes the reduction of methionine-S-sulfoxide to methionine. Our previous study showed that DMSO can competitively inhibit methionine sulfoxide reduction ability of yeast and mammalian MsrA in both in vitro and in vivo, and also act as a non-competitive inhibitor for mammalian MsrB2, specific for the reduction of methionine-R-sulfoxide, with lower inhibition effects. The present study investigated the effects of DMSO on the physiological antioxidant functions of methionine sulfoxide reductases. DMSO elevated hydrogen peroxide-mediated Saccharomyces cerevisiae cell death, whereas it protected human SK-Hep1 cells against oxidative stress. DMSO reduced the protein-carbonyl content in yeast cells in normal conditions, but markedly increased protein-carbonyl accumulation under oxidative stress. Using Msr deletion mutant yeast cells, we demonstrated the DMSO's selective inhibition of the antioxidant function of MsrA in S. cerevisiae, resulting in an increase in oxidative stress-induced cytotoxicity. [BMB reports 2010; 43(9): 622-628]
URI
http://hdl.handle.net/YU.REPOSITORY/23663http://dx.doi.org/10.3858/BMBRep.2010.43.9.622
ISSN
1976-6696
Appears in Collections:
의과대학 > 생화학.분자생물학교실 > Articles
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