Evidence for the role of oxidative stress in the acetylation of histone H3 by ethanol in rat hepatocytes

Title
Evidence for the role of oxidative stress in the acetylation of histone H3 by ethanol in rat hepatocytes
Author(s)
Choudhury M[Choudhury M]박필훈Jackson D[Jackson D]Shukla D[Shukla D]
Keywords
NADPH OXIDASE; GENE-EXPRESSION; LIVER-DISEASE; ALCOHOL; DAMAGE; CELLS; MITOCHONDRIA; INVOLVEMENT; ACTIVATION; MECHANISM
Issue Date
201009
Publisher
ELSEVIER SCIENCE INC
Citation
ALCOHOL, v.44, no.6, pp.531 - 540
Abstract
The relationship between ethanol-induced oxidative stress and acetylation of histone H3 at lysine 9 (H3AcK9) remains unknown and was therefore investigated in primary cultures of rat hepatocytes. Cells were treated with ethanol, and a select group of pharmacological agents and the status of H3AcK9 and reactive oxygen species (ROS) were monitored. Pretreatment of hepatocytes with N-acetyl cystein (ROS reducer), or dietary antioxidants (quercetin, reserveratrol), or NADPH (reduced nicotinamide adenine dinucleotide phosphate) oxidase inhibitor apocynin, significantly reduced ethanol (50 mM, 24 h) induced increases in ROS and H3AcK9. In contrast, L-buthionine sulfoximine (ROS inducer) and inhibitor of mitochondrial complexes I (rotenone) and III (antimycin) increased ethanol-induced H3AcK9 (P < .01). Oxidative stress also affected ethanol-induced alcohol dehydrogenase 1 mRNA expression. These results demonstrate for the first time that oxidative stress is involved in the ethanol-induced histone H3 acetylation in hepatocytes. (C) 2010 Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/23661http://dx.doi.org/10.1016/j.alcohol.2010.06.003
ISSN
0741-8329
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약학대학 > 약학부 > Articles
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