Effect of Platelet-Rich Plasma Concentration on Osteogenic Differentiation of Mesenchymal Stem Cells
- Effect of Platelet-Rich Plasma Concentration on Osteogenic Differentiation of Mesenchymal Stem Cells
- 안면환; 성민철; 조희순; 박세일; 안광욱[안광욱]; 이만호; 박소영; 김석영; 송인환
- platelet rich plasma; mesenchymal stem cell; proliferation and differentiation; osteogenesi
- Issue Date
- 생체재료학회지, v.14, no.3, pp.145 - 150
- Platelet rich plasma (PRP), an autologous platelet concentrate, has been known to potentiate the cellular proliferation depending on the PRP concentration. However, there has been a lot of controversy about the effect of PRP on osteogenic differentiation. This study was performed to clarify the effect of PRP concentration on osteogenic differentiation as well as proliferation of mesenchymal stem cells (MSCs). Bone marrow aspirates and the whole blood were obtained from 6 rabbits for the first part experiment and from 4 human donors for the second part experiment.
MSCs were isolated from the bone marrow aspirates harvested from the iliac crest of rabbit and human. PRP was extracted from the whole blood using a double centrifugation method and activated by thrombin. MSC proliferation and differentiation were analyzed by measuring protein concentration and alkaline phosphatase (ALP) activity which were produced in the culture media. In order to analyze the osteogenic differentiation per cell, ALP/protein ratio was also calculated. The result of in vitro studies using rabbit and human MSCs showed that PRP promotes the proliferative activities of MSCs in a dose-dependent manner. The osteogenic differentiation was suppressed by high concentration of PRP. PRP is also suspected to have a dual effect where low concentration of PRP increases the osteogenic differentiation and high concentration of PRP decreases its differentiation.
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