HER2 Status in Gastric Adenocarcinomas Assessed by Immunohistochemistry, Automated Silver-Enhanced In Situ Hybridization and Fluorescence In Situ Hybridization

Title
HER2 Status in Gastric Adenocarcinomas Assessed by Immunohistochemistry, Automated Silver-Enhanced In Situ Hybridization and Fluorescence In Situ Hybridization
Author(s)
배영경배정민김세원김애리구미진[구미진]
Keywords
HUMAN-BREAST-CANCER; GENE STATUS; PROGNOSTIC-FACTOR; AMPLIFICATION; HER-2/NEU; CARCINOMA; EXPRESSION; ONCOGENE; RECEPTOR
Issue Date
201010
Publisher
KOREAN SOCIETY PATHOLOGISTS
Citation
KOREAN JOURNAL OF PATHOLOGY, v.44, no.5, pp.493 - 501
Abstract
Background : Recently, many studies have focused on human epidermal growth factor receptor 2 (HER2) status in gastric cancer due to HER2-targeted therapy using trastuzumab. We investigated HER2 overexpression and amplification and their concordance rate in Korean gastric adenocarcinomas. Methods : Tissue microarrays were constructed with 232 gastric adenocarcinoma samples. We performed immunohistochemistry (IHC), silver-enhanced in situ hybridization (SISH) and fluorescence in situ hybridization (FISH) for HER2. Results : IHC was negative in 94.8% (218/232), equivocal in 1.7% (4/232) and positive in 3.5% (8/232) of cases. HER2 protein expression was heterogeneous in 75% (9/12) of IHC 2+/3+ cancers. Gene amplification was observed in 6.5% (15/230) by SISH and the same 15 cases were also FISH-positive. We observed HER2 amplification in 1.4%, 27.3%, 25%, and 100% of IHC 0, 1+, 2+, and 3+ gastric adenocarcinomas, respectively. The concordance rate between IHC and SISH results was 95.7%. Conclusions : HER2 overexpression and amplification were less frequent in gastric adenocarcinomas than breast carcinomas. Compared to breast carcinoma, (1) there may be IHC-negative but gene amplification-positive cases for HER2 and (2) frequent intratumoral heterogeneity of IHC for HER2 in gastric adenocarcinomas.
URI
http://hdl.handle.net/YU.REPOSITORY/23522http://dx.doi.org/10.4132/KoreanJPathol.2010.44.5.493
ISSN
1738-1843
Appears in Collections:
의과대학 > 병리학교실 > Articles
의과대학 > 성형외과학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
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