Anti-inflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-kappa B and ERK1/2 inactivation

Title
Anti-inflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-kappa B and ERK1/2 inactivation
Author(s)
장현욱김미화서석종양주혜[양주혜]유예[유예]김수정[김수정]권순렬[권순렬]조태형[조태형]김진원[김진원]박영인[박영인]안계환[안계환]이종길[이종길]김철호[김철호]손종근손건호[손건호]
Keywords
LIPOPOLYSACCHARIDE-INDUCED INOS; ACTIVATED PROTEIN-KINASE; NITRIC-OXIDE SYNTHASE; OCCUPATIONAL ASTHMA; MATRIX-METALLOPROTEINASE-9 EXPRESSION; GENE-EXPRESSION; TNF-ALPHA; ANTIOXIDANT ACTIVITIES; PROSTAGLANDIN E-2; PINELLIA-TERNATA
Issue Date
201010
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.48, no.11, pp.3073 - 3079
Abstract
We identified a bioactive herbal medicine with anti-inflammatory activity from an ethanol extract derived from the bark of Dioscorea batatas DECNE (BDB) in RAW264.7 cells. We examined the effects of BDB on nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production in LPS-induced RAW264.7 cells. BDB consistently inhibited both NO and PGE2 production in a dose-dependent manner, with an IC(50) of 87-71 mu g/ml, respectively. The reduction of NO and PGE2 production were accompanied by a reduction in iNOS and COX-2 protein expression, as evaluated by Western blotting. To evaluate the action mode of BDB and its ability to inhibit iNOS and COX-2 protein expression, we assessed the effects of BDB on nuclear factor-kappa B (NF-kappa B) DNA-binding activity, NF-kappa B-dependent reporter gene activity, inhibitory factor-kappa B (I kappa B) phosphorylation and degradation, and p65 nuclear translocation. BDB suppressed DNA-binding activity and reporter gene activity as well as translocation of the NF-kappa B p65 subunit. BDB also down-regulated I kappa B kinase (IKK), thus inhibiting LPS-induced both phosphorylation and the degradation of I kappa B alpha. In addition, BDB also inhibited the LPS-induced activation of ERK1/2. (C) 2010 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/23503http://dx.doi.org/10.1016/j.fct.2010.07.048
ISSN
0278-6915
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약학대학 > 약학부 > Articles
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