Effect of Lanostane Triterpenes from the Fruiting Bodies of Ganoderma lucidum on Adipocyte Differentiation in 3T3-L1 Cells

Title
Effect of Lanostane Triterpenes from the Fruiting Bodies of Ganoderma lucidum on Adipocyte Differentiation in 3T3-L1 Cells
Author(s)
이익수[이익수]김홍진[김홍진]윤의중[윤의중]김진표[김진표]민병선[민병선]정현주[정현주]나민균Masao Hattori[Masao Hattori]배기환[배기환]
Keywords
INHIBITORY-ACTIVITY; GENE-EXPRESSION; ADIPOGENESIS; CONSTITUENTS; ACIDS; TEA
Issue Date
201010
Publisher
GEORG THIEME VERLAG KG
Citation
PLANTA MEDICA, v.76, no.14, pp.1558 - 1563
Abstract
Two new lanostane triterpenes, methyl lucidenate N (1) and t-butyl lucidenate B (2), were isolated from the fruiting bodies of Ganoderma lucidum together with five known compounds (3-7). The structures of the two new triterpenes were established as methyl 3 beta, 7 beta-dihydroxy-4,4,14 alpha-trimethyl- 11,15-dioxo-5 alpha-chol-8-en-24-oate (1) and t-butyl 7 beta, 12 beta-dihydroxy-4,4,14 alpha-trimethyl-3,11,15-trioxo-5 alpha-chol-8-en-24-oate (2) by extensive spectroscopic studies and chemical evidence. The effect of the isolated compounds (17) on triglyceride (TG) accumulation, an indicator of adipocyte differentiation, during the differentiation of 3T3-L1 preadipocytes was examined. t-Butyl lucidenate B (2) reduced the TG accumulation significantly by 72% at 80 mu M compared to the untreated group. Furthermore, compound 2 effectively suppressed the GPDH activity in the cells. Consistent with the decrease in TG accumulation and GPDH activity, compound 2 suppressed the gene expressions of PPAR gamma, C/EBP alpha, and SREBP-1c in a dose-dependent manner during differentiation. Our findings demonstrate that the lanostane triterpenes isolated in this study contribute to the inhibitory effect of the fruiting bodies of G. lucidum on adipocyte differentiation in 3T3-L1 cells.
URI
http://hdl.handle.net/YU.REPOSITORY/23494http://dx.doi.org/10.1055/s-0030-1249827
ISSN
0032-0943
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약학대학 > 약학부 > Articles
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