Over-expression of Major Royal Jelly Protein 3 from Apis mellifera in E. coli
- Over-expression of Major Royal Jelly Protein 3 from Apis mellifera in E. coli
- 박용하; Phu Van Nguyen[Phu Van Nguyen]; Ji-Na No[Ji-Na No]; Mi-Sun Yoo[Mi-Sun Yoo]; Byoung-Soo Yoon[Byoung-Soo Yoon]
- Apis mellifera; Recombinant protein; MRJP3; Rosetta(DE3)pLysS; IPTG
- Issue Date
- Journal of Apiculture, v.25, no.3, pp.163 - 171
- Major Royal Jelly Protein3 (MRJP3) is a member of Major Royal Jelly Proteins family (MRJPs) that are principal components in Royal Jelly. The MRJP3 was known as potent anti-allergic activity and immune-regulatory or anti-inflammatory. Therefore, the structure and functions of MRJP3 has been studied more and more. In this study, the whole coding sequence of MRJP3 was amplified by using cDNA from Apis mellifera in Korea. The gene was cloned, sequenced and deposited in GenBank (GU434675). It has 99% homology in both nucleotide and amino acid sequence to NM_001011601.
The MRJP3 gene was successfully expressed in both E. coli strain BL21(DE3)pLysS and Rosetta (DE3)pLysS, the yields of recombinant MRJP3 (rMRJP3) were approximately 2mg and 3.5mg protein per liter of the flask culture, respectively. However, we used 40% less IPTG using the Rosetta (DE3)pLysS host which may be important in large scale production of MRJP3. The rMRJP3 will be not only use for generation of monoclonal antibody to develop a qualification tool for RJ but also it might be an useful material for functional research of MRJP3 itself
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