Overexpression of polygalacturonase-inhibiting protein 2 (PGIP2) of Chinese cabbage (Brassica rapa ssp pekinensis) increased resistance to the bacterial pathogen Pectobacterium carotovorum ssp carotovorum

Title
Overexpression of polygalacturonase-inhibiting protein 2 (PGIP2) of Chinese cabbage (Brassica rapa ssp pekinensis) increased resistance to the bacterial pathogen Pectobacterium carotovorum ssp carotovorum
Author(s)
황병호[황병호]배한홍임현섭[임현섭]김군보[김군보]김신제[김신제]임명호[임명호]박범석[박범석]김도선[김도선]김종기[김종기]
Keywords
PHASEOLUS-VULGARIS L; BOTRYTIS-CINEREA; FUNGAL POLYGALACTURONASES; SCLEROTINIA-SCLEROTIORUM; TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; SUBSP CAROTOVORA; GENE-EXPRESSION; NITRIC-OXIDE; APPLE FRUIT
Issue Date
201011
Publisher
SPRINGER
Citation
PLANT CELL TISSUE AND ORGAN CULTURE, v.103, no.3, pp.293 - 305
Abstract
Polygalacturonase-inhibiting proteins (PGIPs)are plant cell wall glycoproteins that can inhibit microbial polygalacturonase (PG) activity. In this study, we cloned five PGIP genes from Chinese cabbage (Brassica rapa ssp. pekinensis). Reverse transcription PCR expression analysis showed that the accumulation of BrPGIP transcripts differed among various tissues and in response to biotic (bacterial innoculation) and abiotic stresses (i.e., wounding, jasmonic acid, cold, NaCl, and dehydration treatment). Transcripts of BrPGIP1, BrPGIP3, and BrPGIP5 were detected in all tissues tested except the stamen, while BrPGIP2 transcripts were expressed in all tissues tested. Transcripts of BrPGIP4 were not expressed in cabbage. lnnoculation with a bacterium that causes soft rot, Pectobacterium carotovorum ssp. carotovorum (Pcc), strongly induced transcripts of BrPGIP2; and expression occurred more rapidly in the resistant compared to the susceptible line. BrPGIP2 showed 50-99% similarity in amino acid sequences to extracellular PGIPs from other plants. In order to assess the role of BrPGIP2 protein in protecting plants from Pcc, we generated a number of transgenic tobacco and Chinese cabbage lines over-expressing BrPGIP2. PGIP from transgenic tobacco plants inhibited Pcc PG activity by 74%, while PGIP from wildtype tobacco plants gave only 43% Pcc PG inhibition. Transgenic Chinese cabbage plants also exhibited improved resistance to bacterial soft rot (up to 54%). PGIP from transgenic Chinese cabbage plants inhibited Pcc PG activity by 77%, while PGIP from wild-type plants showed only 8% Pcc PG inhibition. This is the first report showing the role of BrPGIPs in resistance to bacterial soft rot caused by Pcc.
URI
http://hdl.handle.net/YU.REPOSITORY/23372http://dx.doi.org/10.1007/s11240-010-9779-4
ISSN
0167-6857
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