Usefulness of Frozen-thawed-deglycerolized Red Blood Cells as Quality Control Materials for Red Blood Cell Deformability Test

Title
Usefulness of Frozen-thawed-deglycerolized Red Blood Cells as Quality Control Materials for Red Blood Cell Deformability Test
Author(s)
김유경Suh, Jang Soo[Suh, Jang Soo]Won, Dong Il[Won, Dong Il]Kim, Hyun Ok[Kim, Hyun Ok]Shin, Sehyun[Shin, Sehyun]
Issue Date
201012
Publisher
KOREAN SOC LABORATORY MEDICINE
Citation
KOREAN JOURNAL OF LABORATORY MEDICINE, v.30, no.6, pp.697 - 701
Abstract
Background : The red blood cell (RBC) deformability test is a useful method for measuring the ability of RBCs to adapt their shape to the flow conditions. Using this test, several investigators have shown the relationship between RBC deformability and numerous clinical conditions. For the quality control (QC) of ABC deformability test, we evaluated whether frozen-thawed-deglycerolized RBCs can be used as QC materials. Methods : Packed RBCs were frozen with 40% (wt/vol) glycerol and stored at -80 degrees C for 3 months. For 10 different frozen RBC panels, RBCs were thawed, deglycerolized and stored at 4 degrees C for 4 weeks. Using microfluidic ektacytometer, we measured ABC deformability of the thawed RBCs. The stability of thawed RBCs was tested once a day for 28 days of storage time and was analyzed by simple regression analysis. The precision of the test using thawed RBCs was analyzed for 7 days of storage time by calculation of CV values of intra-assay (10 measurements/assay) and between-day measurements. Results : Frozen-thawed-deglycerolized RBCs were stable for 1 week. Within-run and between-day precisions of the ABC deformability test during 7 days of storage of thawed RBCs were 1.4-2,9%, and 1.9-2.8%, respectively. Conclusions : Frozen-thawed-deglycerolized RBCs used in ABC deformability test showed satisfactory within-run and between-run precisions and stability for one week after thawing, and may be used as QC materials for this test. (Korean J Lab Med 2010;30:697-701)
URI
http://hdl.handle.net/YU.REPOSITORY/23274http://dx.doi.org/10.3343/kjlm.2010.30.6.697
ISSN
1598-6535
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의과대학 > 진단검사의학교실 > Articles
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