A novel method for large-scale immuno-SEM using protein G coupled polystyrene beads

Title
A novel method for large-scale immuno-SEM using protein G coupled polystyrene beads
Author(s)
송인환제임스 데니스[제임스 데니스]
Keywords
SCANNING-ELECTRON-MICROSCOPY; CELLS
Issue Date
201012
Publisher
OXFORD UNIV PRESS
Citation
JOURNAL OF ELECTRON MICROSCOPY, v.59, no.6, pp.527 - 530
Abstract
Polystyrene beads with a mean diameter of 0.76 mu m were coupled with protein G and then anti-type II collagen IgG or anti-chondroitin-4-sulphate IgG were tagged to protein G. Antibody-tagged beads were applied to articular cartilage and labelled beads were counted in each sample. Antibody-tagged beads labelled significantly higher than IgG isotype control. We propose immuno-SEM using protein G coupled beads as a valuable method for micrometre range observation for specific protein distribution on surfaces of tissues or organs. This will provide information about structure as well as antigenicity on the surface at the same time.
URI
http://hdl.handle.net/YU.REPOSITORY/23271http://dx.doi.org/10.1093/jmicro/dfq064
ISSN
0022-0744
Appears in Collections:
의과대학 > 해부학교실 > Articles
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