Modulation of MnSOD protein in response to different experimental stimulation in Hyphantria cunea

Title
Modulation of MnSOD protein in response to different experimental stimulation in Hyphantria cunea
Author(s)
강영진서숙재[서숙재]하남규[하남규]전향미[전향미]한연수[한연수]이인희[이인희]진병래[진병래]김홍자[김홍자]권용민[권용민]김용일[김용일]
Keywords
MANGANESE-SUPEROXIDE-DISMUTASE; PRAWN MACROBRACHIUM-ROSENBERGII; MESSENGER-RNA EXPRESSION; OXIDATIVE STRESS; MOLECULAR-CLONING; DROSOPHILA-MELANOGASTER; REACTIVE OXYGEN; LIFE-SPAN; GRYLLOTALPA-ORIENTALIS; FOOD-DEPRIVATION
Issue Date
201012
Publisher
ELSEVIER SCIENCE INC
Citation
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v.157, no.4, pp.343 - 350
Abstract
A manganese superoxide dismutase (MnSOD) gene was cloned from the fall webworm, Hyphantria cunea. MnSOD cDNAs encode precursor proteins of 215 amino acid residues. H. cunea MnSOD possesses the metal binding ligands of 3 histidines and 1 aspartic acid common to MnSODs. The deduced amino acid sequences of the H. cunea MnSOD cDNA showed 76% identity to Bombyx mori MnSOD and 56-62% identity to MnSOD sequences from other species. MnSOD and copper/zinc superoxide dismutase (Cu/ZnSOD) is expressed in all tissues of H. cunea. MnSOD expression changed at a trace-level in infected larvae, while Cu/ZnSOD expression strongly changed against Gram-positive and Gram-negative bacteria, and fungi. Environmental stresses such as different artificial photoperiods (24L:0D), ultraviolet irradiation (312 nm), and starvation condition increased Cu/ZnSOD expression, MnSOD expression, on the other hand, was increased by starvation. Moreover, MnSOD and Cu/ZnSOD expression showed no significant change in the 0L:24D condition. MnSOD and Cu/ZnSOD expression in H. cunea also significantly increased at high (37 degrees C) and low (4 degrees C) temperature. Oxidative stress induced by 10% H(2)O(2) reduced the expression levels of MnSOD and Cu/ZnSOD. However, paraquat-induced oxidative stress reduced MnSOD expression but increased Cu/ZnSOD expression. These results suggest that Cu/ZnSOD may play a larger role than MnSOD as a superoxide anion scavenger against oxidative stress in H. cunea. (C) 2010 Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/23246http://dx.doi.org/10.1016/j.cbpb.2010.08.003
ISSN
1096-4959
Appears in Collections:
의과대학 > 약리학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE