Determination of Chimera Types and Ploidy Level of Sports from 'Campbell Early' Grape (Vitis labruscana)

Title
Determination of Chimera Types and Ploidy Level of Sports from 'Campbell Early' Grape (Vitis labruscana)
Author(s)
노정호[노정호]박교선[박교선]윤해근도경란[도경란]허윤영[허윤영]김승희[김승희]이한찬[이한찬]류명상[류명상]박서준[박서준]정성민[정성민]
Keywords
NUCLEAR-DNA CONTENT; FLOW CYTOMETRIC ANALYSIS; RAPID-DETERMINATION; PLANTS; COLCHICINE; CULTIVARS; ACTINIDIA; TISSUES; L.
Issue Date
201012
Publisher
KOREAN SOC HORTICULTURAL SCIENCE
Citation
KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY, v.28, no.6, pp.996 - 1002
Abstract
Flow cytometry (FCM) was used to measure the ploidy level of three different sports from 'Campbell Early' (Vitis labruscana) grape. Results of the study showed different ploidy levels. FCM analysis for 'Campbell Early' grape which contains 2C DNA diploid cells showed single peak around 35-40 while 'Kyoho' grape with 4C DNA tetraploid cells had a different level of 70-80. However, analysis of the sports displayed a histogram with 2 peaks containing both 2C and 4C nuclei. There was no difference in histograms of 2C DNA flesh and pericarp; on the other hand, 4C DNA flesh type of sports had a different histogram from that of the 2C DNA pericarp. Chromosome numbers of diploid ('Campbell Early'), tetraploid ('Kyoho'), and three sports were counted under the microscope. 'Campbell Early' and 'Kyoho' have 38 and 76 chromosomes, respectively. Three different sports are mixoploids with mixtures of diploid and tetraploid cells. Microscopic observations of shoot apical meristems in sports from 'Campbell Early' grape were carried out to determine the type of plant chimera. 'Campbell Early' grape (diploid) and 'Kyoho' grape (tetraploid) showed that both had 2 tunica layers covering corpus cells, while the three different sports had tunica layers showing mostly oblique division. Most cells from 'Kyoho' grape were larger than 'Campbell Early' grape. Cells from L-2 and L-3 layers of the three sports were similar to 'Kyoho' grape in size, although all cells in L-1 surface layer were uniform in size like 'Campbell Early' grape. Results of FCM analysis indicated that both normal and polyploid cells could be intermixed in sports and could become mixoploidy consisting of diploid and tetraploid. All sports used in the tests were periclinal chimera plants with two distinct L-1 and L-2 cell layers. The result of this study suggests that all three sports which originated from 'Campbell Early' grape might be 2-4-4 type chimera formation.
URI
http://hdl.handle.net/YU.REPOSITORY/23218
ISSN
1226-8763
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자연자원대학 > 원예생명과학과 > Articles
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