Fabrication of a microarray using a combination of the large circular sense and antisense DNA

Title
Fabrication of a microarray using a combination of the large circular sense and antisense DNA
Author(s)
도경오이윤한[이윤한]한길환[한길환]엄석용[엄석용]김종필[김종필]배윤위[배윤위]박정호[박정호]문익재[문익재]박종구[박종구]
Keywords
DENSITY OLIGONUCLEOTIDE ARRAYS; HUMAN LUNG-CANCER; GENE-EXPRESSION; GROWTH-FACTOR; IN-VITRO; CELLS; TISSUE; SCALE; DISCOVERY; CDNA
Issue Date
201001
Publisher
SPANDIDOS PUBL LTD
Citation
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.25, no.1, pp.113 - 120
Abstract
In the present study, single-stranded large circular (LC)-sense molecules were utilized as probes for DNA microarrays and showed stronger binding signals than those of PCR-amplified cDNA probes. A microarray experiment using 284 LC-sense DNA probes found 6 upregulated and 7 downregulated genes in A549 cells as compared to WI38VA13 cells. Repeated experiments showed largely consistent results, and microarray data strongly correlated with data acquired from quantitative real-time RT-PCR. A large array comprising 5,079 LC-sense DNA was prepared, and analysis of the mean differential expression from dye-swap experiments revealed 332 upregulated and 509 downregulated genes in A549 cells compared to WI38VA13 cells. Subsequent functional analysis using an LC-antisense library of overexpressed genes identified 28 genes involved in A549 cell growth. These experiments demonstrated the proper features of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense and -antisense libraries for an effective functional validation of genes.
URI
http://hdl.handle.net/YU.REPOSITORY/23053http://dx.doi.org/10.3892/ijmm_00000320
ISSN
1107-3756
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의과대학 > 생리학교실 > Articles
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