Enantio selective determination of sibutramine and its active metabolites in human plasma

Title
Enantio selective determination of sibutramine and its active metabolites in human plasma
Author(s)
강원구배경진[배경진]노금한
Issue Date
201001
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.51, no.1, pp.264 - 267
Abstract
Although racemic sibutramine has been widely used for the treatment of obesity, its enantioselective detection method has not been elucidated in human plasma. In this report we introduce a validated analytical method for the determination of sibutramine and its two active metabolites, desmethylsibutramines using LC-MS/MS. R- and S-isomers of those compounds in human plasma were extracted using diethyl ether-hexane (4:1, v/v) followed by an addition of NaOH solution. After removing the organic layer, the residue was reconstituted in the mobile phase 10 mM ammonium acetate solution adjusted to pH 4.0 with acetic acid-acetonitrile (94:6, v/v). Both isomers in the extract were separated on a Chiralcel AGP chiral stationary-phase column and were quantified in a tandem mass spectrometry. The assay method was in accordance with FDA regulations for the validation of bioanalytical methods. This method was successfully used to profile the plasma concentrations of the stereoisomers of sibutramine and its two active metabolites with time in healthy volunteers. (C) 2009 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/23025http://dx.doi.org/10.1016/j.jpba.2009.07.023
ISSN
0731-7085
Appears in Collections:
약학대학 > 약학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE