Effects of Gardeniae Fructus Extract and Geniposide on Promoting Ligament Cell Proliferation and Collagen Synthesis

Title
Effects of Gardeniae Fructus Extract and Geniposide on Promoting Ligament Cell Proliferation and Collagen Synthesis
Author(s)
나민균Quan Cheng Chen [Quan Cheng Chen ]Wei Yun Zhang [Wei Yun Zhang ]김홍진[김홍진]이익수[이익수]Yan Ding [Yan Ding ]윤의중[윤의중]이상명[이상명]민병선[민병선]배기환[배기환]
Keywords
NONSTEROIDAL ANTIINFLAMMATORY DRUGS; SOFT-TISSUE INJURIES; GROWTH-FACTORS; ANTERIOR CRUCIATE; ANKLE; GENIPIN; JASMINOIDES; FIBROBLASTS; EXPLANTS; TENDON
Issue Date
201001
Publisher
JOHN WILEY & SONS LTD
Citation
PHYTOTHERAPY RESEARCH, v.24, pp.S1 - S5
Abstract
Gardeniae Fructus is a traditional medicine used for the treatment of contusion such as ankle sprain. Geniposide is one of the main components of Gardeniae Fructus with diverse biological activities. In order to gain further insight into the therapeutic action of Gardeniae Fructus extract (GFE) and geniposide on ligament injuries, a new in vitro model was developed in the present study. Rat hind ankle ligament fibroblasts (RHALFs) derived from Sprague-Dawley rats were cultured, and the cell proliferation and collagen content were examined by MTT and a Sirius Red-based colorimetric assay after stimulating with each drug. The cell growth of RHALFs was promoted by culturing with 37.5-150 mu g/mL of GFE and 25-200 mu M of geniposide. The content of collagen in the RHALFs was significantly increased up to 131.4% and 124.2% of the control value by culturing with the GFE and geniposide, respectively. By contrast, both cell growth and collagen content were impaired by adding 25-200 mu M of diclofenac, one of the common medications for ligament injuries. The findings suggest that GFE and geniposide may ameliorate the treatment of ligament injuries by proliferating ligament fibroblasts and promoting the synthesis of collagen. However, the use of diclofenac to treat acute ligament injuries should be reassessed although it possesses a potential effect on relieving symptoms. Copyright (C) 2009 John Wiley & Sons, Ltd.
URI
http://hdl.handle.net/YU.REPOSITORY/23005http://dx.doi.org/10.1002/ptr.2839
ISSN
0951-418X
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약학대학 > 약학부 > Articles
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