Development of Polyclonal Antibodies to Abdominal and Subcutaneous Adipocyte for Producing Fat-reduced High Quality Pork

Title
Development of Polyclonal Antibodies to Abdominal and Subcutaneous Adipocyte for Producing Fat-reduced High Quality Pork
Author(s)
최창본최창원[최창원]백경훈오영균[오영균]홍성구[홍성구]
Keywords
PLASMA-MEMBRANES; CHICKEN ADIPOCYTES; ANTISERUM; TISSUE; DEPOSITION; INVITRO; GROWTH; INVIVO
Issue Date
201002
Publisher
KOREAN SOC FOOD SCIENCE ANIMAL RESOURCES
Citation
KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES, v.30, no.1, pp.87 - 94
Abstract
The aim of the present study was to develop polyclonal antibodies to regional inedible adipocytes of pigs and investigate the effect of these antibodies on adipocytes in vitro As antigens, abdominal and subcutaneous adipocyte PMPs from pigs were injected into sheep 3 times per 3 wk intervals for passive immunization, and non-immunized serum, antisera against abodominal (AAb) or subcutaneous adipocyte PMPs (SAb) were collected before and after the injections, Titers of the antisera obtained from sheep and then cross-reactivities with the heart. kidney. liver. lung, muscle. and spleen of pig, were determined by ELISA Isolation and culture of abdominal and subcutaneous adipocytes from pigs were performed to analyze LDH concentration At a 1 1,000 dilution, little antibody reactivity was observed for non-immunized serum whereas both AAb and SAb had relatively strong reactivity up to a dilution of 1 16,000. These findings may indicate that strong antibodies against adipocyte PMPs can be developed using an immunological approach Extremely low reactivity of AAb and SAb was detected with the PMPs of the organs. Both antisera most strongly reacted with each adipocyte PMPs and showed statistically (p<0 05) higher cross-reactivities compared with the non-immunized serum In conclusion, these results may indicate that file present polyclonal antibodies against regional Inedible adipocyte PMPs are well developed and are safe against cross-reactivities with the organs of pigs Further studies on the in vivo nutritional safety and fat reduction of these antibodies in pigs will be required fat-reduced high quality pork production
URI
http://hdl.handle.net/YU.REPOSITORY/22958
ISSN
1225-8563
Appears in Collections:
생명공학부 > 생명공학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE