Sevoflurane-induced post-conditioning has no beneficial effects on neuroprotection after incomplete cerebral ischemia in rats

Title
Sevoflurane-induced post-conditioning has no beneficial effects on neuroprotection after incomplete cerebral ischemia in rats
Author(s)
이덕희이혜미[이혜미]최준혁이성룡[이성룡]김용운지대림도현석[도현석]이호명[이호명]박석주[박석주]
Keywords
REDUCES INFARCT SIZE; REPERFUSION INJURY; NEURONAL DAMAGE; NITRIC-OXIDE; IN-VIVO; ISOFLURANE; HALOTHANE; PROPOFOL; DESFLURANE; APOPTOSIS
Issue Date
201002
Publisher
WILEY-BLACKWELL PUBLISHING, INC
Citation
ACTA ANAESTHESIOLOGICA SCANDINAVICA, v.54, no.3, pp.328 - 336
Abstract
Background The aim of this study was to investigate whether sevoflurane-induced post-conditioning has a neuroprotective effect against incomplete cerebral ischemia in rats. Methods After cerebral ischemia by right common carotid artery occlusion in combination with hemorrhagic hypotension (35 mmHg) for 30 min, 1.0 minimum alveolar concentration of sevoflurane was administered for 15 min (Post-C 15, n=8), 30 min (Post-C 30, n=8), or 60 min (Post-C 60, n=8) in rats. Sevoflurane was not administered in control (n=8) and sham control rats (n=8). Neurologic evaluations were performed at 24, 48, and 72 h after ischemia. Degrees of neuronal damage in ischemic hippocampal CA1 and the cortex were assessed by counting eosinophilic neurons, and detection of DNA fragmentation was performed by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining. Results Neurologic deficit scores in the Post-C 60 group were higher than in the control group at 48 and 72 h post-ischemia (P < 0.05). No differences were observed in the percentages of eosinophilic neurons among the control (CA1: 37.3 +/- 25.4, cortex: 26.0 +/- 8.9), Post-C 15 (CA1: 54.0 +/- 21.4, cortex: 30.8 +/- 19.9), or Post-C 30 (CA1: 68.4 +/- 17.5, cortex: 38.0 +/- 11.0) groups in ischemic CA1 and cortices. However, in the Post-C 60 group, the percentages of eosinophilic neurons were higher than in the control group in CA1 and cortices (P < 0.05). The percentages of TUNEL-positive cell were similar in the control group and the post-conditioned groups. Conclusion These findings show that sevoflurane administration after ischemia does not provide neuroprotection in rats subjected to incomplete cerebral ischemia.
URI
http://hdl.handle.net/YU.REPOSITORY/22957http://dx.doi.org/10.1111/j.1399-6576.2009.02129.x
ISSN
0001-5172
Appears in Collections:
의과대학 > 마취통증의학교실 > Articles
의과대학 > 병리학교실 > Articles
의과대학 > 생리학교실 > Articles
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