Grifola frondosa water extract alleviates intestinal inflammation by suppressing TNF-alpha production and its signaling

Title
Grifola frondosa water extract alleviates intestinal inflammation by suppressing TNF-alpha production and its signaling
Author(s)
김정애이종숙박수영따파디네쉬최미경[최미경]정일민[정일민]박영준[박영준]용철순최한곤
Keywords
TUMOR-NECROSIS-FACTOR; FACTOR-KAPPA-B; EPITHELIAL-CELL LINES; ACID-INDUCED COLITIS; TNBS-INDUCED COLITIS; BOWEL-DISEASE; CROHNS-DISEASE; CHEMOKINE EXPRESSION; INDUCED ACTIVATION; OXIDATIVE STRESS
Issue Date
201002
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Citation
EXPERIMENTAL AND MOLECULAR MEDICINE, v.42, no.2, pp.143 - 154
Abstract
TNF-alpha is a major cytokine involved in inflammatory bowel disease (IBD). In this study, water extract of Grifola frondosa (GFW) was evaluated for its protective effects against colon inflammation through the modulation of TNF-alpha action. In coculture of HT-29 human colon cancer cells with U937 human monocytic cells, TNF-alpha-induced monocyte adhesion to HT-29 cells was significantly suppressed by GFW (10, 50, 100 mu g/ml). The reduced adhesion by GFW correlated with the suppressed expression of MCP-1 and IL-8, the major IBD-associated chemokines. In addition, treatment with GFW significantly suppressed TNF-alpha-induced reactive oxygen species production and NF-kappa B transcriptional activity in HT-29 cells. In differentiated U937 monocytic cells, LPS-induced TNF-alpha production, which is known to be mediated through NF-kappa B activation, was significantly suppressed by GFW. In an in vivo rat model of IBD, oral administration of GFW for 5 days (1 g/kg per day) significantly inhibited the trinitrobenzene sulfonic acid (TNBS)-induced weight loss, colon ulceration, myeloperoxidase activity, and TNF-alpha expression in the colon tissue. Moreover, the effect of GFW was similar to that of intra-peritoneal injection of 5-aminosalicylic acid (5-ASA), an active metabolite of sulfasalazine, commonly used drug for the treatment of IBD. The results suggest that GFW ameliorates colon inflammation by suppressing production of TNF-alpha as well as its signaling through NF-kappa B leading to the expression of inflammatory chemokines, MCP-1 and IL-8. Taken together, the results strongly suggest GFW is a valuable medicinal food for IBD treatment, and thus may be used as an alternative medicine for IBD.
URI
http://hdl.handle.net/YU.REPOSITORY/22951http://dx.doi.org/10.3858/emm.2010.42.2.016
ISSN
1226-3613
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약학대학 > 약학부 > Articles
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