Design and Application of an Inertial Impactor in Combination with an ATP Bioluminescence Detector for In Situ Rapid Estimation of the Efficacies of Air Controlling Devices on Removal of Bioaerosols

Title
Design and Application of an Inertial Impactor in Combination with an ATP Bioluminescence Detector for In Situ Rapid Estimation of the Efficacies of Air Controlling Devices on Removal of Bioaerosols
Author(s)
변정훈윤기영[윤기영]박철우[박철우]황정호[황정호]
Keywords
SIZE DISTRIBUTIONS; AIRBORNE MICROBES; MICROORGANISMS; ENUMERATION; AEROSOLS; ASSAY
Issue Date
201003
Publisher
AMER CHEMICAL SOC
Citation
ENVIRONMENTAL SCIENCE & TECHNOLOGY, v.44, no.5, pp.1742 - 1746
Abstract
We proposed a rapid method to estimate the efficacies of air controlling devices in Situ using ATP bioluminescence in combination with an inertial impactor. The inertial impactor was designed to have 1 mu m of cutoff diameter, and its performance was estimated analytically, numerically, and experimentally. The proposed method was characterized using Staphylococcus epidermidis, which was aerosolized with a nebulizer. The bioaerosol concentrations were estimated within 25 min using the proposed method without a culturing process, which requires several days for colony formation. A linear relationship was obtained between the results of the proposed ATP method (RLU/m(3)) and the conventional culture-based method (CFU/m(3)), with R(2) 0.9283. The proposed method was applied to estimate the concentration of indoor bioaerosols, which were identified as a mixture of various microbial species including bacteria, fungi, and actinomycetes, in an occupational indoor environment, controlled by mechanical ventilation and an air cleaner. Consequently, the proposed method showed a linearity with the culture-based method for indoor bioaerosols with R(2) 0.8189, even though various kinds of microorganisms existed in the indoor air. The proposed method may be effective in monitoring the changes of relative concentration of indoor bioaerosols and estimating the effectiveness of air control devices in indoor environments.
URI
http://hdl.handle.net/YU.REPOSITORY/22780http://dx.doi.org/10.1021/es903437z
ISSN
0013-936X
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공과대학 > 기계공학부 > Articles
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