Full metadata record

DC FieldValueLanguage
dc.contributor.author비시누 판데이[비시누 판데이]ko
dc.contributor.author노창현[노창현]ko
dc.contributor.author최권영[최권영]ko
dc.contributor.author이나훔[이나훔]ko
dc.contributor.author김은정[김은정]ko
dc.contributor.author고성기[고성기]ko
dc.contributor.author김태진[김태진]ko
dc.contributor.author윤형돈ko
dc.contributor.author김병기[김병기]ko
dc.date.accessioned2015-12-17T00:07:16Z-
dc.date.available2015-12-17T00:07:16Z-
dc.date.created2015-11-13-
dc.date.issued201003-
dc.identifier.citationBIOTECHNOLOGY AND BIOENGINEERING, v.105, no.4, pp.697 - 704-
dc.identifier.issn0006-3592-
dc.identifier.urihttp://hdl.handle.net/YU.REPOSITORY/22779-
dc.identifier.urihttp://dx.doi.org/10.1002/bit.22582-
dc.description.abstractRegiospecific 3'-hydroxylation reaction of daidzein was performed with CYP105D7 from Streptomyces avermitilis MA4680 expressed in Escherichia coli. The apparent K(m) and k(cat) values of CYP105D7 for daidzein were 21.83 +/- 6.3 mu M and 15.01 +/- 0.6 min(-1) in the presence of 1 mu M of CYP105D7, putidaredoxin (CamB) and putidaredoxin reductase (CamA), respectively. When CYP105D7 was expressed in S. avermitilis MA4680, its cytochrome P450 activity was confirmed by the CO-differente spectra at 450 nm using the whole cell extract. When the whole-cell reaction for the 3'-hydroxylation reaction of daidzein was carried out with 100 mu M of daidzein in 100 mM of phosphate buffer (pH 7.5), the recombinant S. avermitilis grown in R2YE media overexpressing CYP105D7 and ferredoxin FdxH (SAV7470) showed a 3.6-fold higher conversion yield (24%) than the corresponding wild type cell (6.7%). In a 7 L (working volume 3 L) jar fermentor, the recombinants S. avermitilis grown in R2YE media produced 112.5 mg of 7,3',4'-trihydroxyisoflavone (i.e., 29.5%, conversion yield) from 381 mg of daidzein in 15 h. Biotechnol. Bioeng. 2010;105: 697-704. (C) 2009 Wiley Periodicals Inc.-
dc.language영어-
dc.publisherWILEY-BLACKWELL-
dc.subjectISOFLAVONES DAIDZEIN-
dc.subjectIN-VITRO-
dc.subjectINHIBITOR-
dc.subjectGENISTEIN-
dc.titleRegioselective Hydroxylation of Daidzein Using P450 (CYP105D7) From Streptomyces avermitilis MA4680-
dc.typeArticle-
dc.identifier.wosid000274742200004-
dc.identifier.scopusid2-s2.0-77449114845-
Appears in Collections:
생명공학부 > 생명공학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE