Bile tolerant Lactobacillus reuteri isolated from pig feces inhibits enteric bacterial pathogens and porcine rotavirus

Title
Bile tolerant Lactobacillus reuteri isolated from pig feces inhibits enteric bacterial pathogens and porcine rotavirus
Author(s)
박용하서병주[서병주]문미란[문미란]레지쉬쿠마르김철중[김철중]장영효[장영효]이인선[이인선]
Keywords
LACTIC-ACID BACTERIA; PROBIOTIC LACTOBACILLUS; ANTIBIOTIC SUSCEPTIBILITY; ACIDOPHILUS; VACCINATION; DIARRHEA; HEALTH; BACTERIOTHERAPY; GASTROENTERITIS; RESISTANCE
Issue Date
201003
Publisher
SPRINGER
Citation
VETERINARY RESEARCH COMMUNICATIONS, v.34, no.4, pp.323 - 333
Abstract
Lactic acid producing bacterial strain Probio-16 was isolated from the swine excrements under anaerobic conditions and characterized by morphology and biochemical characteristics. The strain was further identified by 16S rRNA gene sequencing and phylogeneitc analysis. The antimicrobial activity of the strain was assayed by testing for growth inhibition of thirteen pathogenic microorganisms. The strain was tested for antiviral activity against porcine rotavirus in vitro in African green monkey epithelial cell line TF-104. Antibiotic susceptibility of the strain against 13 antibiotics was tested using disk diffusion method. Phenotypically and through 16S rRNA gene sequences, Probio-16 was identified and named as Lactobacillus reuteri Probio-16. This strain was resistant to pH 2.0, 5% porcine bile and exhibited antimicrobial activity against all the thirteen enteric bacterial pathogens tested. Probio-16 supernatant inhibited porcine rotavirus in vitro in TF-104 cell lines. Except for erythromycin and penicillin G at a concentration of 4 mu g/ml, Probio-16 showed resistance to all other thirteen antibiotics tested. This study indicates L. reuteri Probio-16 as a novel strain with its tolerance to low pH and bile, antimicrobial activity, antibiotic resistance and antiviral activity against rotavirus, and an ideal probiotic candidate for animal and human application after the proper in vivo experiments.
URI
http://hdl.handle.net/YU.REPOSITORY/22773http://dx.doi.org/10.1007/s11259-010-9357-6
ISSN
0165-7380
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생명공학부 > 생명공학부 > Articles
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