장환형 단일가닥 DNA를 이용한 암세포 성장 억제 유전자 발굴

Title
장환형 단일가닥 DNA를 이용한 암세포 성장 억제 유전자 발굴
Other Titles
Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes.
Author(s)
배윤위[배윤위]문익재[문익재]서영배[서영배]도경오
Keywords
Microarray; expression profiling; microarray; antisense; lung cancer
Issue Date
201003
Publisher
한국미생물·생명공학회
Citation
한국미생물·생명공학회지, v.38, no.1, pp.70 - 76
Abstract
The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarray and antisense libraries for an effective functional validation of genes.
URI
http://hdl.handle.net/YU.REPOSITORY/22714
ISSN
1598-642X
Appears in Collections:
의과대학 > 생리학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE