Protein G와 NHS를 이용하여 고정한 anti-IgG의 검출 성능 비교

Title
Protein G와 NHS를 이용하여 고정한 anti-IgG의 검출 성능 비교
Other Titles
A comparison of detection capabilities of anti-IgG immobilizedby protein G and NHS
Author(s)
손영호손영수[손영수]신은정[신은정]
Keywords
protein G; self-assembled monolayer; surface plasmon resonance; immunosensor
Issue Date
201003
Publisher
한국센서학회
Citation
센서학회지, v.19, no.2, pp.142 - 148
Abstract
We have compared and investigated the detection capabilities of antibody of immunoglobulin G(anti-IgG) immobilized by protein G and N-hydroxysuccinimide(NHS) at the end of the self-assembled monolayer(SAM). Surface plasmon resonance(SPR) sensor has been utilized to measure the interaction between biomolecules. After formation of the protein G and SAM, anti-IgG, bovine serum albumin(BSA) and IgG has been sequently injected. Through the reponse of the SPR, we can conclude that the protein G immobilized anti-IgG better than the SAM. In addition, IgG detection capability of the anti-IgG immobilized by the protein G showed better performance compared with that immobilized by the SAM.
URI
http://hdl.handle.net/YU.REPOSITORY/22688
ISSN
1225-5475
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공과대학 > 컴퓨터공학과 > Articles
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