Mutation Spectra Induced by Adozelesin in the supF Gene of Human XP-A Fibroblasts

Title
Mutation Spectra Induced by Adozelesin in the supF Gene of Human XP-A Fibroblasts
Author(s)
이종순이세영[이세영]Gerd P. Pfeifer[Gerd P. Pfeifer]
Keywords
ANTITUMOR ANTIBIOTIC CC-1065; DNA-SEQUENCE SPECIFICITY; ESCHERICHIA-COLI; MAMMALIAN-CELLS; SHUTTLE VECTOR; ADDUCT; POLYMERASE; INVITRO; ALKYLATION; NSC-298223
Issue Date
201004
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.33, no.4, pp.633 - 636
Abstract
Adozelesin is a synthetic analog of the antitumor antibiotic, CC-1065, which alkylates N3 of adenine in the minor DNA groove in a sequence-specific manner. Here we tested the mutation spectra induced by adozelesin in the supF gene of human XP-A fibroblasts using a shuttle vector assay. Adozelesin primarily induces mutations via an A -> T transversion and a single base insertion. The A -> T transversion (43/59) was observed at the adenine alkylation site in the 5'-ATTTA* sequence (A* is the site of alkylation). The single base insertion (5/59) was observed at the 3'-side of the covalently modified adenine in the 5'-CTAAA* sequence. The results of this study suggest that the DNA alkylating sequence of adozelesin influences the type of DNA mutation.
URI
http://hdl.handle.net/YU.REPOSITORY/22599http://dx.doi.org/10.1007/s12272-010-0419-7
ISSN
0253-6269
Appears in Collections:
이과대학 > 화학생화학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE