Expression of thermostable bacterial beta-glucosidase (BglB) in transgenic tobacco plants

Title
Expression of thermostable bacterial beta-glucosidase (BglB) in transgenic tobacco plants
Author(s)
정세라[정세라]김수연[김수연]배한홍임현섭[임현섭]배현종[배현종]
Keywords
CELLULOLYTICUS ENDOGLUCANASE E1; RECOMBINANT PROTEINS; THERMOTOGA-MARITIMA; AUTOHYDROLYSIS; TRANSFORMATION; PURIFICATION; PRETREATMENT; HYDROLYSIS; BIOETHANOL; BIOFUELS
Issue Date
201004
Publisher
ELSEVIER SCI LTD
Citation
BIORESOURCE TECHNOLOGY, v.101, no.18, pp.7144 - 7150
Abstract
Transgenic tobacco plants expressing the hyperthermostable B-glucosidase BglB of Thermotoga mamma were generated with the goal of cost-effective production of the enzyme for the application in bioconversion of lignocellulosic biomass. The enzyme was targeted to the cytosol and chloroplasts, where it accumulated to level of 4.5% and 5.8% of total soluble protein, respectively The optimal temperature and pH of the plant-expressed BglB was 80 degrees C and 4.5, respectively. BglB activity was preserved in leaves after lyophilization, but decreased by over 70% with drying at room temperature When BglB was synergistically supplied in a 1% (w/v) rice straw with Cel5A for efficient cellulase conversion, a 37% increase in glucose was observed This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB. (C) 2010 Elsevier Ltd All rights reserved
URI
http://hdl.handle.net/YU.REPOSITORY/22586http://dx.doi.org/10.1016/j.biortech.2010.03.140
ISSN
0960-8524
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생명공학부 > 생명공학부 > Articles
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