A functional carbohydrate chip platform for analysis of carbohydrate-protein interaction

Title
A functional carbohydrate chip platform for analysis of carbohydrate-protein interaction
Author(s)
서정현김창섭황병희[황병희]차형준[차형준]
Keywords
SELF-ASSEMBLED MONOLAYERS; CHOLERA-TOXIN; SUGAR CHAINS; OLIGOSACCHARIDE MICROARRAYS; BINDING; SURFACE; IMMOBILIZATION; GLYCOSYLATION; SPECIFICITY; RECEPTOR
Issue Date
201005
Publisher
IOP PUBLISHING LTD
Citation
NANOTECHNOLOGY, v.21, no.21
Abstract
A carbohydrate chip based on glass or other transparent surfaces has been suggested as a potential tool for high-throughput analysis of carbohydrate-protein interactions. Here we proposed a facile, efficient, and cost-effective method whereby diverse carbohydrate types are modified in a single step and directly immobilized onto a glass surface, with retention of functional orientation. We modified various types of carbohydrates by reductive amination, in which reducing sugar groups were coupled with 4-(2-aminoethyl)aniline, which has di-amine groups at both ends. The modified carbohydrates were covalently attached to an amino-reactive NHS-activated glass surface by formation of stable amide bonds. This proposed method was applied for efficient construction of a carbohydrate microarray to analyze carbohydrate-protein interactions. The carbohydrate chip prepared using our method can be successfully used in diverse biomimetic studies of carbohydrates, including carbohydrate-biomolecule interactions, and carbohydrate sensor chip or microarray development for diagnosis and screening.
URI
http://hdl.handle.net/YU.REPOSITORY/22476http://dx.doi.org/10.1088/0957-4484/21/21/215101
ISSN
0957-4484
Appears in Collections:
공과대학 > 화학공학부 > Articles
이과대학 > 화학생화학부 > Articles
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