A functional carbohydrate chip platform for analysis of carbohydrate-protein interaction
- A functional carbohydrate chip platform for analysis of carbohydrate-protein interaction
- 서정현; 김창섭; 황병희[황병희]; 차형준[차형준]
- SELF-ASSEMBLED MONOLAYERS; CHOLERA-TOXIN; SUGAR CHAINS; OLIGOSACCHARIDE MICROARRAYS; BINDING; SURFACE; IMMOBILIZATION; GLYCOSYLATION; SPECIFICITY; RECEPTOR
- Issue Date
- IOP PUBLISHING LTD
- NANOTECHNOLOGY, v.21, no.21
- A carbohydrate chip based on glass or other transparent surfaces has been suggested as a potential tool for high-throughput analysis of carbohydrate-protein interactions. Here we proposed a facile, efficient, and cost-effective method whereby diverse carbohydrate types are modified in a single step and directly immobilized onto a glass surface, with retention of functional orientation. We modified various types of carbohydrates by reductive amination, in which reducing sugar groups were coupled with 4-(2-aminoethyl)aniline, which has di-amine groups at both ends. The modified carbohydrates were covalently attached to an amino-reactive NHS-activated glass surface by formation of stable amide bonds. This proposed method was applied for efficient construction of a carbohydrate microarray to analyze carbohydrate-protein interactions. The carbohydrate chip prepared using our method can be successfully used in diverse biomimetic studies of carbohydrates, including carbohydrate-biomolecule interactions, and carbohydrate sensor chip or microarray development for diagnosis and screening.
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