Effect of Sex Steroid Hormones on Bovine Myogenic Satellite Cell Proliferation, Differentiation and Lipid Accumulation in Myotube

Title
Effect of Sex Steroid Hormones on Bovine Myogenic Satellite Cell Proliferation, Differentiation and Lipid Accumulation in Myotube
Author(s)
최인호이은주프라티바즈라촤리여장은정장종수[장종수]이현정[이현정]홍성구[홍성구]
Keywords
SKELETAL-MUSCLE CELLS; GENE-EXPRESSION; TRENBOLONE-ACETATE; ESTROGEN-RECEPTORS; GROWTH-FACTOR; IGF-I; ANDROGEN; MICE; TESTOSTERONE; HYPERTROPHY
Issue Date
201005
Publisher
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC
Citation
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, v.23, no.5, pp.649 - 658
Abstract
Myogenic satellite cells (MSCs) are adult stem cells that activate and differentiate into myotubes. These stem cells are multipotent as they transdifferentiate into adipocyte-like cells, nerve cells and osteocytes. The effects of steroid hormones (E(2) and testosterone) were studied as a further step toward understanding the mechanism of MSCs proliferation and differentiation. In this study, MSCs were grown continuously for 87 days, implying that there may be a group of MSCs that continue to proliferate rather than undergoing differentiation. Isolated MSCs were cultured in Dulbecco's Modified Eagle's Medium supplemented with adult male, female or castrated bovine serum to observe the effect of steroid hormones on MSC proliferation. Cell proliferation was the highest in cultures supplemented with male serum followed by female and castrated serum. The positive effect of male hormone on MSC proliferation was confirmed by the observation of testosterone-mediated increased proliferation of cells cultured in medium supplemented with castrated serum. Furthermore, steroid hormone treatment of MSCs increased lipid accumulation in myotubes. Oil-Red-O staining showed that 17 beta-estradiol (E(2)) treatment avidly increased lipid accumulation, followed by E(2)+testosterone and testosterone alone. To our knowledge, this is the first report of lipid accumulation in myotubes due to steroids in the absence of an adipogenic environment, and the effect of steroid hormones on cell proliferation using different types of adult bovine serum, a natural hormonal system. In conclusion, we found that sex steroids affect MSCs proliferation and differentiation, and lipid accumulation in myotubes.
URI
http://hdl.handle.net/YU.REPOSITORY/22466
ISSN
1011-2367
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생명공학부 > 생명공학부 > Articles
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