Cysteine-125 is the catalytic residue of Saccharomyces cerevisiae free methionine-R-sulfoxide reductase

Title
Cysteine-125 is the catalytic residue of Saccharomyces cerevisiae free methionine-R-sulfoxide reductase
Author(s)
김화영곽근희[곽근희]김문정[김문정]
Keywords
GAF DOMAIN; PROTEINS; MAMMALS; YEAST; EXPRESSION; REPAIR
Issue Date
201005
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.395, no.3, pp.412 - 415
Abstract
Free methionine-R-sulfoxide reductase (fRMsr) is a new type of methionine sulfoxide reductase that catalyzes the reduction of free methionine-R-sulfoxide to methionine. This enzyme cannot reduce oxidized methionine residues in proteins. While three Cys residues, Cys-91, Cys-101 and Cys-125, have been demonstrated to be involved in the catalysis by Saccharomyces cerevisiae fRMsr, their specific functions have not been fully established. In this work, we performed in vivo growth complementation experiments using S. cerevisiae cells lacking all three known methionine sulfoxide reductases. Cells containing a C1255 construct, in which Cys-125 in fRMsr was replaced with Ser, did not grow in methionine sulfoxide medium, whereas cells containing C915, C1015, or C91/1015 constructs could grow in this medium. In addition, when assayed with thioredoxin and glutaredoxin reduction systems, the C1255 form was inactive, whereas C915 and C1015 had 1-2% and 9-10%, respectively, of the activity of the wild-type fRMsr. These data show that Cys-125 is the catalytic residue in fRMsr. (C) 2010 Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/22463http://dx.doi.org/10.1016/j.bbrc.2010.04.036
ISSN
0006-291X
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의과대학 > 생화학.분자생물학교실 > Articles
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