Protective effects of Ulmus davidiana var. japonica against OVA-induced murine asthma model via upregulation of heme oxygenase-1

Title
Protective effects of Ulmus davidiana var. japonica against OVA-induced murine asthma model via upregulation of heme oxygenase-1
Author(s)
손종근이미영[이미영]서창섭[서창섭]하혜경[하혜경]Jung D[Jung D]Lee H,[Lee H,]Lee NH[Lee NH]Lee JA[Lee JA]Kim JH[Kim JH]이연경Shin HK[ Shin HK]
Keywords
INDUCED AIRWAY INFLAMMATION; PLANCH ULMACEAE; ALLERGIC-ASTHMA; SENSITIZED RATS; LUNG INJURY; T-CELLS; PROTEIN; HYPERRESPONSIVENESS; HYPERREACTIVITY; INDUCTION
Issue Date
201006
Publisher
ELSEVIER IRELAND LTD
Citation
JOURNAL OF ETHNOPHARMACOLOGY, v.130, no.1, pp.61 - 69
Abstract
Aim of the study: Traditionally, the stem and root bark of Ulmus davidiana var. japonica (Ulmaceae) are Korean herbal medicines used for anti-inflammatory and anticancer therapy. In this study, we investigated the protective effects of Ulmus davidiana var. japonica ethanolic extract (UD) in a murine asthma model. Furthermore, we determined whether heme oxygenase (HO)-1 is required for the protective activity of UD. Materials and methods: Airways of ovalbumin (OVA)-sensitized mice exposed to OVA challenge developed eosinophilia, mucus hypersecretion and increased cytokine levels. UD was applied 1 h prior to OVA challenge. Mice were administered UD orally at doses of 100 and 200 mg/kg once daily on days 18-23. Bronchoalveolar lavage fluid (BALF) was collected 48 h after the final OVA challenge. Levels of interleukin (IL)-4 and IL-5 in BALF were measured using enzyme-linked immunosorbent assays (ELISAs). Lung tissue sections 4 mu m in thickness were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration and mucus production with PAS (periodic acid shift reagent) staining, in conjunction with ELISA, immunohistochemistry and Western blot analyses for HO-1 protein expression. Results and conclusion: Orally administered UD significantly inhibited the number of OVA-induced inflammatory cells and IgE production, along with reduced T-helper (Th)2 cytokine levels, such as IL-4 and IL-5, in BALF and lung tissue. In addition, UD induced a marked decrease in OVA-induced reactive oxygen species (ROS), inflammatory cell infiltration and mucus production in lung tissue. These effects were correlated with HO-1 mRNA and protein induction. Our results indicate that UD protects against OVA-induced airway inflammation, at least in part, via HO-1 upregulation. Crown Copyright (C) 2010 Published by Elsevier Ireland Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/22335http://dx.doi.org/10.1016/j.jep.2010.04.011
ISSN
0378-8741
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약학대학 > 약학부 > Articles
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